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首页> 外文期刊>Zoological Science >In Vitro Differentiation of Chicken Astrocytes: Growth, Morphology, and Protein Expression of Astrocytes in Primary Cultures
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In Vitro Differentiation of Chicken Astrocytes: Growth, Morphology, and Protein Expression of Astrocytes in Primary Cultures

机译：鸡星形胶质细胞的体外分化：原发性培养物中星形胶质细胞的生长，形态和蛋白质表达

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摘要

Astrocytes regulate synaptic transmission in the central nervous system. Astrocytes in vivo have "stems" that express glial fibrillary acidic protein (GFAP), intermediate filaments, and peripheral astrocyte processes (PAPs), which contain actin-rich cytoskeletal structures. At the PAPs, the perisynaptic glia contacts and enwraps synapses, and modulates glia-neuronal communication. Cultured astrocytes have been an invaluable tool for studying roles of astrocytes; however, the morphology of mammalian primary astrocytes cultured in conventional medium containing fetal bovine serum (FBS) was similar to that of fibroblasts, and many culture conditions have been developed to generate stellate astrocytes observed in vivo. Avian astrocytes have been prepared from embryonic chick forebrain and maintained at a high cell density in conventional FBS-containing medium as mammalian astrocytes, thus the morphological analysis of chicken astrocytes has not yet been performed. In the present study, we report that the morphology of astrocytes freshly harvested from the forebrain of a chicken embryo in serum-free Neurobasal medium with B-27 supplement and basic fibroblast growth factor (bFGF) is similar to that of the astrocyte morphology in vivo. We also find that astrocytes in this medium express similar levels of GFAP and two actin-binding proteins as astrocytes in conventional FBS-containing medium, although they have different morphologies. Furthermore, we confirmed that cryopreserved astrocytes differentiate faster than freshly harvested astrocytes.
机译：星形胶质细胞调节中枢神经系统中的突触传递。体内的星形胶质细胞具有表达胶质纤维酸性蛋白（GFAP），中间细丝和外周星形胶质细胞方法（PAPS）的“茎”，其含有富含致动蛋白的细胞骨架结构。在PAPS，蠕动胶质胶片触点和突触突触，并调制胶质胶神经元通信。培养的星形胶质细胞是学习星形胶质细胞的角色的宝贵工具;然而，在含有胎牛血清（FBS）的常规培养基中培养的哺乳动物原代星形胶质细胞的形态与成纤维细胞的形态相似，并且已经开发出许多培养条件以产生在体内观察到的星状星形胶质细胞。已经从胚胎小鸡前脑制备禽的星形胶质细胞，并在含常规FBS的培养基中保持高细胞密度作为哺乳动物星形胶质细胞，因此尚未进行鸡星形胶质细胞的形态学分析。在本研究中，我们报告说，从B-27补充剂和碱性成纤维细胞生长因子（BFGF）的无血清神经蛋白酶中，新鲜从鸡胚的前脑进行新鲜收获的星形胶质细胞的形态类似于体内星形胶质细胞形态的血清神经蛋白酶培养基。我们还发现该培养基中的星形胶质细胞表达了类似的GFAP和两种肌动蛋白结合蛋白作为常规FBS培养基中的星形胶质细胞，尽管它们具有不同的形态。此外，我们证实冷冻保存的星形胶质细胞比新鲜收获的星形胶质细胞更快。

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