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首页> 外文期刊>Zoological Science >ECM-body: A Cell-free 3D Biomimetic Scaffold Derived From Intact Planarian Body
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ECM-body: A Cell-free 3D Biomimetic Scaffold Derived From Intact Planarian Body

机译：ECM-Body：一种无关的3D仿生支架，来自完整的平面体

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摘要

Extracellular matrix (ECM) plays key roles in shaping fates of stem cells, not only by providing a suitable niche but also by mediating physical and biochemical cues. Despite intensive investigations on regeneration, the roles of ECM in fate determination of stem cells in animals with great regenerative potency, such as planarian, have remained unclear. Here, we developed a method for decellularizing and isolating extracellular matrix from planarians. Although the isolated scaffold appears translucent, it contains all the internal features resembling those of the structure of intact planarians, and we thus called it the "ECM-body". Nuclear staining demonstrated that the ECM-body contains very few or no remaining cells. Histological sections displayed well-preserved morphological integrity of the specimen. Scanning electron microscopy showed a porous surface on the ECM-body, potentially suitable for housing cells. Furthermore, our preliminary experiment suggested that ECM-body can be utilized as a biomimetic scaffold for cell culture as it may support survival of injected neoblasts.
机译：细胞外基质（ECM）在干细胞的成形率中起关键作用，不仅通过提供合适的利基而且通过介导物理和生化线索。尽管对再生进行了密集的调查，但ECM在具有巨大再生效力（如Planian的动物中干细胞的作用仍然尚不清楚。在这里，我们开发了一种从平面图中脱节和分离细胞外基质的方法。虽然孤立的脚手架出现半透明，但它包含了类似于完整平面图的结构的所有内部特征，因此我们称之为“ECM-Body”。核染色证明ECM-体含有很少或没有剩余细胞。组织学部门显示出样品的完全保存的形态完整性。扫描电子显微镜显示ECM-体上的多孔表面，可能适用于容纳电池。此外，我们的初步实验表明ECM-体可以用作细胞培养的仿真支架，因为它可能支持注射新细胞的存活。

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