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首页> 外文期刊>Zoological Science >The Stomatogastric and Enteric Nervous System of the Pulmonate Snail Megalobulimus abbreviatus: A Neurochemical Analysis
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The Stomatogastric and Enteric Nervous System of the Pulmonate Snail Megalobulimus abbreviatus: A Neurochemical Analysis

机译:肺蜗牛蜂霉素蜂胶蜂鸟类腹部腹腔内和肠道神经系统:神经化学分析

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摘要

Chemical coding of stomatogastric nervous system (STNS) and enteric nervous system (ENS) of midgut and hindgut in the snail Megalobulimus abbreviatus was investigated using histochemistry, histofluorescence, and immunohistochemistry. The gastrointestinal plexuses, constituted by intrinsic neurons and fibers originating from the subesophageal ganglia and/or STNS, showed intense acetylcholinesterase (AChE) and nicotinamide adenine dinucleotide diaphorase (NADPHd) activity. The enteric neurons and fibers with AChE activity are scattered in the submucosa and between both muscular layers of gastrointestinal tract, whereas NADPHd neurons and fibers are more abundant between muscular layers than in the submucosa. Catecholaminergic nerve fibers and varicosities are found mainly within the submucosa across the mid- and hindgut. Serotoninand FMRFamide-immunoreactive neurons and fibers originating from the STNS are distributed in the submucosa of the intestine and rectum. FMRFamide-immunoreactive neurons and fibers are present in the mucosa, submucosa, and muscular layers of mid-and hindgut. The neuron-like intraepithelial cells exhibited AChE activity, a few NADPHd activity, and immunoreactivity for serotonin and FMRFamide. Intense glial fibrillary acidic protein (GFAP) immunoreaction is found throughout the intestine plexuses and in the STNS ganglia. The GFAP immunoreaction in intramural plexuses suggests the presence of glial cells as an important component of ENS in this pulmonate snail.
机译:使用组织化学,组织组织化学,组织荧光和免疫组化研究了蜗牛MegAlobulimus Abbreviatus的中肠和肠道神经系统(STN)和肠道神经系统(ENS)的化学编码。由源自沉积神经节和/或STN的内在神经元和纤维构成的胃肠道丛显示强烈的乙酰胆碱酯酶(ACHE)和烟酰胺腺嘌呤二核苷酸透明酶(NADPHD)活性。肠道神经元和纤维具有疼痛活动散落在粘膜下,并且在胃肠道两层之间分散,而NADPHD神经元和纤维在肌肉层之间比粘膜下肌肉层更丰富。在中间和后肠的粘膜下发现,儿茶酚胺能神经纤维和致血清。血清素和FMRFamide-免疫反应性神经元和源自STN的纤维分布在肠和直肠的粘膜下。 MMRFamide-ImmunoreActive神经元和纤维存在于中和后肠的粘膜,粘膜下和肌肉层中。类似神经元的上皮细胞表现出疼痛的活性,少数NADPHD活性,以及​​血清素和FMRFamide的免疫反应性。在整个肠道神经丛和Stns Ganglia中发现强烈的胶质纤维酸性蛋白(GFAP)免疫反应。 Intramury Plexuses中的GFAP免疫反应表明,在该肺蜗牛中,存在胶质细胞作为ENS的重要组成部分。

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