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首页> 外文期刊>Zoological Science >Morphogenesis and Mucus Production of Epithelial Tissues of Three Major Salivary Glands of Embryonic Mouse in 3D Culture
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Morphogenesis and Mucus Production of Epithelial Tissues of Three Major Salivary Glands of Embryonic Mouse in 3D Culture

机译：三种培养胚胎小鼠三大唾液腺体上皮组织的形态发生和粘液生产

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摘要

Embryonic mouse submandibular epithelia initiate branching morphogenesis within two days when embedded in Matrigel and stimulated by members of the epidermal growth factor family. However, it is unknown whether the end buds further branch over longer culture periods, and whether saliva-producing cells differentiate there. In the present study, we cultivated three major (submandibular, sublingual and parotid) salivary epithelia from 13-day embryos for 14 days in mesenchyme-free cultures. All epithelia continued to grow and branch to form numerous acinus-like structures in medium supplemented with neuregulin 1, fibroblast growth factor 1, and insulintransferrin-sodium selenite. Alcian blue staining to detect mucous cells showed that each epithelium differentiated via three distinct modes, as seen in normal development, although the staining intensities were weaker than in normal development. RT-PCR analysis of the amylase gene showed that no epithelia expressed amylase after 14 days of culture, which is inconsistent with the fact that only parotid epithelium does so at postnatal day 7 during normal development. These results suggest that cytodifferentiation progresses to a lesser extent in mesenchyme-free cultures than in vivo.
机译：胚胎小鼠颌下颌骨上皮内上皮发生在嵌入Matrigel中的两天内，并由表皮生长因子家族的成员刺激。然而，尚不清楚末端芽是否进一步分支超过更长的培养期，以及唾液产生细胞是否区分。在本研究中，我们在不含间充细胞培养物中培养了从13天胚胎的三个主要（颌骨，舌下和腮腺）唾液上皮细胞14天。所有上皮均继续生长和分支，以在补充有Neuregulin 1，成纤维细胞生长因子1和胰岛素中的培养基中形成许多类似的Aginus样结构。 Alcian Blue染色以检测粘液细胞显示每种上皮通过三种不同模式分化，如正常发育中所见，尽管染色强度弱于正常发育。淀粉酶基因的RT-PCR分析显示，在14天的培养后没有上皮表达淀粉酶，这与在正常发育期间仅在后期第7天在后期第7天这样的事实不一致。这些结果表明，细胞质细胞预期在不含间充质的培养物中的程度越小，而不是体内。

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