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首页> 外文期刊>Biotechnology Progress >Effects of three-dimensional culturing in a fibrous matrix on cell cycle, apoptosis, and MAb production by hybridoma cells
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Effects of three-dimensional culturing in a fibrous matrix on cell cycle, apoptosis, and MAb production by hybridoma cells

机译：在纤维基质中三维培养对杂交瘤细胞周期，凋亡和单克隆抗体产生的影响

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摘要

The effects of culturing hybridoma cells in a three-dimensional (3-D) poly(ethylene terephthalate) (PET) fibrous matrix on cell cycle, apoptosis, metabolism, and monoclonal antibody (MAb) production were evaluated by comparing with two-dimensional (2-D) culturing on microcarrier and multiwell plate surfaces. The percentage of cells in the G1/G0 phase increased during the long-term culturing period of similar to4 weeks. Compared to the 2-D culture systems, cells grown in 3-D matrices had higher MAb productivity for long-term culture. Decreasing serum content in the culture medium increased both MAb productivity and apoptosis. However, the 3-D culture had a greater increase in MAb productivity and a much lower apoptotic rate than the 2-D culture, especially at 0% serum. Most cells in the 3-D fibrous matrix formed large aggregates and were smaller than cells grown on a 2-D surface or in suspension. The smaller cell size allowed cells to survive better in the high-cell-density environment. The fibrous matrix also selectively retained healthy, nonapoptotic cells. These results suggested that the 3-D fibrous matrix contributed to growth arrest, protected cells to better resist low-serum environments, and reduced apoptosis, all of which contributed to the high viable cell density and volumetric MAb productivity in the long-term 3-D culture.
机译：通过与二维（3-D）聚对苯二甲酸乙二酯（PET）纤维基质中培养杂交瘤细胞对细胞周期，凋亡，代谢和单克隆抗体（MAb）产生的影响进行评估。 2-D）在微载体和多孔板表面上培养。在大约4周的长期培养期间，G1 / G0期的细胞百分比增加。与2-D培养系统相比，在3-D基质中生长的细胞具有更高的MAb长期培养生产力。培养基中血清含量的减少会同时提高单克隆抗体的生产率和细胞凋亡。但是，3-D培养物的MAb生产率提高了很多，而凋亡率却比2-D培养物低得多，尤其是在0％血清条件下。 3-D纤维基质中的大多数细胞形成大的聚集体，并且比在2D表面或悬浮液中生长的细胞小。较小的细胞大小可使细胞在高细胞密度环境中更好地存活。纤维基质还选择性保留健康的非凋亡细胞。这些结果表明，3-D纤维基质可促进生长停滞，保护细胞更好地抵抗低血清环境，并减少细胞凋亡，所有这些都有助于长期提高细胞密度和提高单克隆抗体的生产能力3- D文化。

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