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首页> 外文期刊>Zygote >Stereological analysis of gonads from diploid and triploid fish yellowtail tetra Astyanax altiparanae (Garutti & Britski) in laboratory conditions
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Stereological analysis of gonads from diploid and triploid fish yellowtail tetra Astyanax altiparanae (Garutti & Britski) in laboratory conditions

机译：实验室条件下二倍体和三倍体鱼黄尾鱼黄尾鱼黄尾鱼黄土（Garutti＆Britski）的立体学分析

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This study aimed to examine the gonadal morphology of diploid and triploid fish through stereological analysis. Triploid individuals were obtained after temperature shock (40 degrees C for 2 min) at 2 min post-fertilization and reared until 175 days post-fertilization (dpf). Intact eggs were used to obtain the diploids. Gonads were collected for histological analysis at 83, 114, 144 and 175 dpf. Diploid females and males presented normal oogenesis and spermatogenesis through all the experimental period. Conversely, stereological analysis revealed that triploid females were sterile and oogonia were the prevalent cell type in the ovaries. Triploid males presented increased amounts of spermatocyte cysts and a large area of lumen when compared with diploids and in addition the amount of spermatozoa was lower than that observed for diploids. However, some triploid males presented spermatogenesis similar to diploids. Therefore, we concluded that triploidization is an interesting alternative to produce sterile individuals in A. altiparanae.
机译：本研究旨在通过立体学分析来研究二倍体和三倍体鱼的Gonadal形态。在施用后2分钟后温度冲击（40摄氏度持续2分钟）获得三倍体个体，并饲养施用后175天（DPF）。完整卵用于获得二倍体。在83,114,144和175 dpf下收集Gonads进行组织学分析。二倍体女性和男性通过所有实验期呈现正常的oferoises和精子发生。相反，立体学分析表明，三倍体女性是无菌的，卵黄素是卵巢中的普遍细胞类型。与二倍体相比，三倍体雄性呈现增加的精子细胞囊肿和大面积的内腔，并且精子的量低于二倍体观察到的精子。然而，一些三倍体男性呈现出类似的分脂剂。因此，我们得出结论，三倍化是在A. Altiparanae中产生无菌个体的有趣替代品。

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