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Effect of washing on yield in one- and two-step steam pretreatment of softwood for production of ethanol

机译：一两步蒸汽预处理软木生产乙醇中洗涤对收率的影响

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Two-step steam pretreatment of softwood on laboratory scale has previously been shown to result in higher yields than one-step steam pretreatment. In this study, these results are verified on a larger scale. In an industrial process filtration and washing of the material between the two pretreatment steps are difficult without release of pressure. A worst case without filtration or washing was thus investigated to determine the influence of poor washing on the yield of sugars and the formation of byproducts. Steam pretreatment with SO2 impregnation was investigated using three different procedures. One-step steam pretreatment was performed at 215 degreesC for 5 min. Two different kinds of two-step steam pretreatment were performed at 190 degreesC for 2 min in the first step and at 210 degreesC for 5 min in the second step. In one case the slurry obtained after the first pretreatment step was separated into a liquid and a solid phase, where the water-insoluble solid material was washed with water and then used for pretreatment in the second step. In the other case of two-step steam pretreatment, neither separation nor washing was performed. The pretreated material was evaluated using both enzymatic hydrolysis and fed-batch simultaneous saccharification and fermentation. Both two-step steam pretreatment process configurations investigated resulted in higher yields of ethanol (300 L/ton) than one-step steam pretreatment (227 L/ton). Separation and washing of the material between the pretreatment steps in the two-step steam pretreatment process did not improve the overall sugar yield, although the formation of sugar degradation products was reduced.
机译：实验室规模的软木两步蒸汽预处理已被证明比单步蒸汽预处理具有更高的产量。在这项研究中，这些结果得到了更大范围的验证。在工业过程中，在不释放压力的情况下，很难在两个预处理步骤之间过滤和洗涤材料。因此研究了没有过滤或洗涤的最坏情况，以确定不良洗涤对糖收率和副产物形成的影响。使用三种不同的方法研究了用SO2浸渍进行蒸汽预处理的方法。在215摄氏度下进行一步蒸汽预处理5分钟。第一步在190℃下进行2分钟，第二步在210℃下进行5分钟，进行两种不同的两步蒸汽预处理。在一种情况下，在第一预处理步骤之后获得的浆料被分离成液相和固相，其中水不溶性固体材料用水洗涤，然后用于第二步骤中的预处理。在两步蒸汽预处理的另一种情况下，既不进行分离也不进行洗涤。使用酶促水解和补料分批同时糖化和发酵来评估预处理的材料。研究的两步蒸汽预处理工艺配置均比一步蒸汽预处理（227 L /吨）产生更高的乙醇产率（300 L /吨）。尽管减少了糖降解产物的形成，但是在两步蒸汽预处理过程中，在预处理步骤之间进行材料的分离和洗涤并没有提高总糖产量。

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