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首页> 外文期刊>Biotechnology Progress >Modeling two-component isoelectric focusing buffers in a vortex-stabilized electrophoresis apparatus
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Modeling two-component isoelectric focusing buffers in a vortex-stabilized electrophoresis apparatus

机译：在涡流稳定电泳仪中模拟两组分等电聚焦缓冲液

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Defined buffer systems have been considered by researchers as replacements for synthetic carrier ampholyte mixtures in IEF in order to reduce both costs and deleterious protein-ampholyte interactions. In this paper, a mathematical model for the behavior of two-component pH gradients during IEF is applied to a vortex-stabilized electrophoresis apparatus. Equations for mass conservation, molar fluxes, and the electric field describe the behavior of the components in the electric field. Equilibrium constants are used in the model to account for interconversion between the positive, negative, and neutral states for each component. The model was applied to predict pH gradients using two different defined buffer systems. The model's predictions fell within the 95% confidence interval of a least-squares fit to the experimental pH gradients except in the regions near the ends of the pH gradients, where large pH excursions occurred during the experiments.
机译：研究人员认为，定义的缓冲液系统可替代IEF中的合成载体两性电解质混合物，以降低成本和有害的蛋白质-两性电解质相互作用。在本文中，将用于IEF期间两组分pH梯度行为的数学模型应用于涡旋稳定电泳仪。质量守恒，摩尔通量和电场的方程式描述了电场中各组分的行为。在模型中使用平衡常数来说明每个组件的正，负和中性状态之间的相互转换。使用两个不同的定义缓冲系统，将该模型应用于预测pH梯度。该模型的预测值落在与实验pH梯度拟合的最小二乘法的95％置信区间内，但在pH梯度末端附近的区域除外，在实验过程中发生了较大的pH漂移。

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