• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Solvent-free enzymatic synthesis of 1,3-diconjugated linoleoyl glycerol optimized by response surface methodology
【24h】

Solvent-free enzymatic synthesis of 1,3-diconjugated linoleoyl glycerol optimized by response surface methodology

机译：响应面法优化的无溶剂酶促合成1,3-二共轭亚油酰甘油

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An operation mode with N-2 bubbling under vacuum was employed for the solvent-free synthesis of 1,3-diconjugated linoleoyl glycerol (1,3-dCLG) from conjugated linoleic acid (CLA) catalyzed by Novozym 435. The response surface methodology (RSM) was adopted for the optimization of the reaction conditions with five major factors (incubation time, temperature, enzyme load, substrate mole ratio, and system vacuum) and three responses (CLA conversion, 1,3-dCLG yield, and acyl migration). Two sets of optimal conditions were recommended. Validation of the RSM model was verified by the good agreement between the experimental and the predicted values of 1,3-dCLG yield. Under the optimal conditions, the yield of 1,3-dCLG up to 93% was obtained. The reaction was scaled up to a production level of 100 g of 1,3-dCLG at a yield of 90.7%, indicating a promising feature of the technology in industrial applications.
机译：真空下N-2鼓泡的操作模式用于由Novozym 435催化从共轭亚油酸（CLA）无溶剂合成1,3-二共轭亚油酰甘油（1,3-dCLG）。响应面方法（采用RSM）通过五个主要因素（孵育时间，温度，酶负载，底物摩尔比和系统真空）和三个响应（CLA转化率，1,3-dCLG收率和酰基迁移）来优化反应条件。建议使用两组最佳条件。通过实验与1,3-dCLG产量的预测值之间的良好一致性来验证RSM模型的有效性。在最佳条件下，获得了高达93％的1,3-dCLG。将该反应放大至100 g 1,3-dCLG的生产水平，产率为90.7％，表明该技术在工业应用中具有令人鼓舞的特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号