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Hollow-fiber enzyme reactor operating under nonisothermal conditions

机译：在非等温条件下运行的中空纤维酶反应器

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摘要

A hollow-fiber enzyme reactor, operating under isothermal and nonisothermal conditions, was built employing a polypropylene hollow fiber onto which beta-galactosidase was immobilized. Hexamethylenediamine and glutaraldehyde were used as spacer and coupling agent, respectively. Glucose production was studied as a function of temperature, substrate concentration, and size of the transmembrane temperature gradient. The actual average temperature differences across the polypropylene fiber, to which reference was done to evaluate the effect of the nonisothermal conditions, were calculated by means of a mathematical approach, which made it possible to know, using computer simulation, the radial and axial temperature profiles inside the bioreactor and across the membrane. Percent activity increases, proportional to the size of the temperature gradients, were found when the enzyme activities under nonisothermal conditions were compared to those measured under comparable isothermal conditions. Percent reductions of the production times, proportional to the applied temperature gradients, were also calculated. The advantage of employing nonisothermal bioreactors in biotechnological industrial process was discussed.
机译：使用在其上固定有β-半乳糖苷酶的聚丙烯中空纤维构建在等温和非等温条件下运行的中空纤维酶反应器。六亚甲基二胺和戊二醛分别用作间隔基和偶联剂。研究了葡萄糖产生与温度，底物浓度和跨膜温度梯度大小的关系。聚丙烯纤维上的实际平均温差（通过参考以评估非等温条件的影响）是通过数学方法计算的，该方法可以使用计算机模拟来了解径向和轴向温度曲线在生物反应器内部并跨膜。将非等温条件下的酶活性与可比的等温条件下测得的酶活性进行比较，发现活性百分率与温度梯度的大小成正比。还计算了与所应用的温度梯度成比例的生产时间减少的百分比。讨论了在生物技术工业过程中使用非等温生物反应器的优势。

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