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首页> 外文期刊>Human fertility: journal of the British Fertility Society >The effects of different sperm preparation methods and incubation time on the sperm DNA fragmentation.
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The effects of different sperm preparation methods and incubation time on the sperm DNA fragmentation.

机译：不同精子制备方法的影响和孵育时间对精子DNA碎裂。

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摘要

PURPOSE: To study the effect of different sperm preparation methods and incubation times post preparation on sperm DNA fragmentation. METHODS: Sperm DNA fragmentation was assessed by sperm chromatin dispersion (SCD) method on motile sperm prepared by gradient centrifugation or swim-up and incubated in IVF medium for up to 24 hours. Data were analyzed to discover the effect of preparation methods and incubation times on sperm DNA Fragmentation Index (DFI). RESULTS: There were no differences in DFI in sperm samples prepared by gradient centrifugation method or swim-up (3.87 +/- 2.14 vs. 3.45 +/- 1.83, p = 0.544). However, an increase was observed in DFI in samples prepared by swim-up after 6-8 hours compared with by gradient centrifugation (34 degrees vs. 13 degrees , p = 0.04). In the swim-up group, the DFI level at 4 hours was already significantly higher than it was initially. However, following gradient centrifugation, the DFI at 8 hours was significantly higher than the initial DFI level. CONCLUSION: Sperm samples prepared by gradient centrifugation may be more stable compared to samples prepared by swim-up in terms of DNA Fragmentation.
机译：目的：研究不同精子制备方法的影响和孵育时间在精子DNA碎片中的制备。方法：通过梯度离心或泳列制备的运动精子上的精子染色质分散体（SCD）方法评估精子DNA碎片，并在IVF培养基中孵育长达24小时。分析数据以发现制备方法和孵育时间对精子DNA碎片指数（DFI）的效果。结果：通过梯度离心方法或扫描制备的精子样品中的DFI中没有差异（3.87 +/- 2.14与3.45 +/- 1.83，P = 0.544）。然而，在通过梯度离心（34度与13度，P = 0.04）中，在6-8小时后，在6-8小时后，在通过扫描制备的样品中的样品中观察到增加。在筹集组中，4小时的DFI水平已经明显高于最初的程度。然而，在梯度离心之后，8小时的DFI显着高于初始DFI水平。结论：与通过在DNA碎裂方面制备的样品相比，通过梯度离心制备的精子样品可以更稳定。

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