• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Effect of Oxygen Limitation and Medium Composition on Escherichia coli Fermentation in Shake-Flask Cultures
【24h】

Effect of Oxygen Limitation and Medium Composition on Escherichia coli Fermentation in Shake-Flask Cultures

机译：限制氧和培养基组成对烧瓶培养中大肠杆菌发酵的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Shake-flask cultures are widely used for screening of high producing strains.To select suitable strains for production scale,cultivation parameters should be applied that provide optimal growth conditions.A novel method of measuring respiratory activity in shake-flask cultures was employed to analyze Escherichia coli fermentation under laboratory conditions.Our results suggest that the length of fermentation,choice of medium,and aeration do not normally satisfy the requirements for unlimited growth in shake flasks.Using glycerol rather than glucose as a carbon source greatly reduced the accumulation of overflow and fermentative metabolites when oxygen supply was unlimited.A rich buffered medium,Terrific Broth (TB),yielded 5 times more biomass compared to LB medium but also caused oxygen limitation in standard shake-flask cultures at shaking frequencies below 400 rpm.These results were used to optimize the production of benzoylformate decarboxylase from Pseudomonas putida in E.coli SG13009,resulting in a 10-fold increase in volumetric enzyme production.This example demonstrates how variation of medium composition and oxygen supply can be evaluated by the measurement of the respiratory activity.This can help to efficiently optimize screening conditions for E.coli.
机译：摇瓶培养物被广泛用于筛选高产菌株。为了选择适合生产规模的菌株，应采用能提供最佳生长条件的培养参数。采用一种测量摇瓶培养物呼吸活性的新方法来分析大肠杆菌。结果表明，发酵时间，培养基选择和通气通常不能满足摇瓶无限生长的要求。使用甘油而不是葡萄糖作为碳源可大大减少溢流和累积当氧气供应无限时，发酵代谢产物。丰富的缓冲液，特异的肉汤（TB）产生的生物量是LB培养基的5倍，但在低于400 rpm的振荡频率下也导致标准摇瓶培养中的氧气受限。优化大肠杆菌SG13中恶臭假单胞菌苯甲酰甲酸酯脱羧酶的生产009，使酶的体积产量增加了10倍。该示例说明了如何通过测量呼吸活动来评估培养基组成和氧气供应的变化。这有助于有效地优化大肠杆菌的筛选条件。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号