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首页> 外文期刊>Biotechnology Progress >Effects of Sulfuric Acid Loading and Residence Time on the Composition of Sugarcane Bagasse Hydrolysate and Its Use as a Source of Xylose for Xylitol Bioproduction
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Effects of Sulfuric Acid Loading and Residence Time on the Composition of Sugarcane Bagasse Hydrolysate and Its Use as a Source of Xylose for Xylitol Bioproduction

机译：硫酸负载量和停留时间对甘蔗渣水解产物组成的影响及其作为木糖醇生物生产中木糖来源的用途

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摘要

A 2~2 full factorial design was employed to evaluate the effects of sulfuric acid loading and residence time on the composition of sugarcane bagasse hydrolysate obtained in a 250-L reactor.The acid loading and the residence time were varied from 70 to 130 mg acid per gram of dry bagasse and from 10 to 30 min,respectively,while the temperature (121 deg C) and the bagasse loading (10%) were kept constant.Both the sulfuric acid loading and the residence time influenced the concentrations of xylose and inhibitors in the hydrolysate.The highest xylose concentration (22.71 g/L) was achieved when using an acid loading of 130 mg/g and a residence time of 30 min.These conditions also led to increased concentrations of inhibiting byproducts in the hydrolysate.All of the hydrolysates were vacuum-concentrated to increase the xylose concentration,detoxified by pH alteration and adsorption into activated charcoal,and used for xylitol bioproduction in a stirred tank reactor.Neither the least (70 mg/g,10 min) nor the most severe (130 mg/g,30 min) hydrolysis conditions led to the best xylitol production (37.5 g/L),productivity (0.85 g/L h),and yield (0.78 g/g).
机译：采用2〜2的全因子设计来评估硫酸负载量和停留时间对250 L反应器中获得的甘蔗渣水解产物组成的影响，酸负载量和停留时间在70到130 mg酸之间变化每克干甘蔗渣分别在10至30分钟内保持恒定温度（121摄氏度）和甘蔗渣含量（10％）。硫酸含量和停留时间均影响木糖和抑制剂的浓度当使用130 mg / g的酸负载量和30分钟的停留时间时，最高木糖浓度（22.71 g / L）达到了这些条件，这些条件也导致了水解产物中抑制副产物的浓度增加。将水解产物真空浓缩以增加木糖浓度，通过改变pH值解毒并吸附到活性炭中，并在搅拌釜反应器中用于木糖醇的生物生产。至少（70 mg / g 10分钟）或最苛刻的水解条件（130 mg / g，30分钟）导致最佳的木糖醇产量（37.5 g / L），生产率（0.85 g / L h）和产量（0.78 g / g）。

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