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Heuristic Optimization of Antibody Production by Chinese Hamster Ovary Cells

机译：中国仓鼠卵巢细胞抗体生产的启发式优化

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摘要

Large-scale production of monoclonal antibodies necessitates the development of a commercially viable process using the appropriate bioreactors.,culture medium,and optimal feeding strategies.In the development of feeding strategies for higher antibody titers it is critical to assess the effects of limiting substrates on cell culture longevity and antibody production.In this study,glucose and L-glutamine were identified as limiting substrates and their effects on culture longevity and antibody production were evaluated in small-scale experiments.The results suggested that an optimal feeding strategy should account for the osmolality profile of the culture.The heuristic approach taken to optimize the antibody production showed that the fed-batch cultivation is superior to batch culture and maintaining low osmolality during growth phase increases cumulative viable cell density and thus leads to higher final antibody titer.
机译：单克隆抗体的大规模生产需要使用合适的生物反应器，培养基和最佳的进料策略来开发商业上可行的方法。在开发更高抗体滴度的进料策略中，评估限制底物对抗体的作用至关重要。在这项研究中，葡萄糖和L-谷氨酰胺被鉴定为限制性底物，并在小规模实验中评估了它们对培养寿命和抗体产生的影响。结果表明，最佳的喂养策略应考虑到培养物的重量克分子渗透率分布图。采用启发式方法优化抗体产量显示，分批补料培养优于批次培养，在生长期保持低克分子渗透压可增加累积的活细胞密度，从而导致更高的最终抗体滴度。

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