...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>AJRI: American Journal of Reproductive Immunology >Granulocyte-Macrophage Colony-Stimulating-Factor Increases Interferon-tau Protein Secretion in Bovine Trophectoderm Cells.
【24h】

Granulocyte-Macrophage Colony-Stimulating-Factor Increases Interferon-tau Protein Secretion in Bovine Trophectoderm Cells.

机译：粒细胞-巨噬细胞集落刺激因子增加牛滋养外胚层细胞中干扰素-tau蛋白的分泌。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Problem Uterine-derived factors are required for optimal conceptus development and secretion of the maternal recognition of pregnancy factor, interferon-tau (IFN-tau). Identifying these factors may lead to the development of schemes for increasing pregnancy success in cattle. Method of Study The objectives were to examine the effects of granulocyte-macrophage colony-stimulating-factor (GM-CSF) on trophectoderm proliferation rates and IFN-tau production, and verify the appropriateness of using an in vitro model of bovine trophectoderm (CT-1 cell). Results Rate of [(3)H]-thymidine incorporation into DNA was increased by supplementation of CT-1 medium with 10 or 100 ng/mL porcine (po) GM-CSF. GM-CSF supplementation to CT-1 medium also increased IFN-tau secretion. When results were normalized to account for number of CT-1 cells, 10 and 100 ng/mL poGM-CSF increased antiviral activity and IFN-tau concentrations (using an IFN-tau-specific enzyme-linked immunosorbent assay) in CT-1 conditioned medium compared with controls. Conclusions These findings indicate that GM-CSF increases proliferation and IFN-tau production in bovine trophectoderm.
机译：问题子宫衍生因子是最佳概念发育和分泌孕妇对妊娠因子干扰素-tau（IFN-tau）的认识所必需的。识别这些因素可能会导致开发提高牛妊娠成功率的方案。研究方法目的是研究粒细胞巨噬细胞集落刺激因子（GM-CSF）对滋养外胚层增殖速率和IFN-tau产生的影响，并验证使用牛滋养外胚层体外模型（CT- 1个单元格）。结果通过向CT-1培养基中添加10或100 ng / mL猪（po）GM-CSF，可以提高[（3）H]-胸苷掺入DNA的速率。 GM-CSF对CT-1培养基的补充也增加了IFN-tau的分泌。当将结果标准化为考虑到CT-1细胞的数量时，在CT-1条件下，10和100 ng / mL poGM-CSF会增加抗病毒活性和IFN-tau浓度（使用IFN-tau特异性酶联免疫吸附测定）与对照组相比为中等。结论这些发现表明，GM-CSF可增加牛滋养外胚层的增殖和IFN-tau的产生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号