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首页> 外文期刊>Biotechnology Progress >Synthesis of Novel Porous Magnetic Silica Microspheres as Adsorbents for Isolation of Genomic DNA
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Synthesis of Novel Porous Magnetic Silica Microspheres as Adsorbents for Isolation of Genomic DNA

机译：新型多孔磁性二氧化硅微球的合成作为基因组DNA分离的吸附剂

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摘要

An improved procedure is described for preparation of novel mesoporous microspheres consisting of magnetic nanoparticles homogeneously dispersed in a silica matrix.The method is based on a three-step process,involving (i) formation of hematite/silica composite microspheres by urea-formaldehyde polymerization,(ii) calcination of the composite particles to remove the organic constituents,and (iii) in situ transformation of the iron oxide in the composites by hydrogen reductive reaction.The as-synthesized magnetite/silica composite microspheres were nearly monodisperse,mesoporous,and magnetizable,with as typical values an average diameter of 3.5 mu m,a surface area of 250 m~2/g,a pore size of 6.03 nm,and a saturation magnetization of 9.82 emu/g.These magnetic particles were tested as adsorbents for isolation of genomic DNA from Saccharomyces cerevisiae cells and maize kernels.The results are quite encouraging as the magnetic particle based protocols lead to the extraction of genomic DNA with satisfactory integrity,yield,and purity.Being hydrophilic in nature,the porous magnetic silica microspheres are considered a good alternative to polystyrene-based magnetic particles for use in biomedical applications where nonspecific adsorption of biomolecules is to be minimized.
机译：描述了一种改进的制备介孔微球的方法，该介孔微球由均匀分散在二氧化硅基质中的磁性纳米粒子组成。该方法基于三步法，涉及（i）通过脲醛聚合形成赤铁矿/二氧化硅复合微球， （ii）煅烧复合颗粒以去除有机成分，以及（iii）通过氢还原反应原位转变复合物中的氧化铁。合成后的磁铁矿/二氧化硅复合微球几乎单分散，介孔且可磁化作为典型值，平均直径为3.5μm，表面积为250 m〜2 / g，孔径为6.03 nm，饱和磁化强度为9.82 emu / g。测试了这些磁性颗粒作为分离剂的吸附剂从酿酒酵母细胞和玉米粒中提取基因组DNA。结果令人鼓舞，因为基于磁性颗粒的方案导致提取基因组DNA具有令人满意的完整性，产率和纯度。多孔磁性二氧化硅微球本质上具有亲水性，被认为是生物医学应用中聚苯乙烯基磁性颗粒的良好替代品，其中生物分子的非特异性吸附应降至最低。

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