• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Differential Gene Expression Profiles and Real-Time Measurements of Growth Parameters in Saccharomyces cerevisiae Grown in Microliter-Scale Bioreactors Equipped with Internal Stirring
【24h】

Differential Gene Expression Profiles and Real-Time Measurements of Growth Parameters in Saccharomyces cerevisiae Grown in Microliter-Scale Bioreactors Equipped with Internal Stirring

机译：配备内部搅拌的微升规模生物反应器中生长的酿酒酵母的差异基因表达谱和生长参数的实时测量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Combining real-time growth kinetics measurements with global gene expression analysis of microbial cultures is of significant value for high-throughput biological research.We have performed differential gene expression analysis in the eukaryotic model Saccharomyces cerevisiae grown in galactose and glucose media in 150 mu L bioreactors equipped with sensors for in situ and real-time measurements of optical density (OD),pH,and dissolved oxygen (DO).The microbioreactors were fabricated from poly(dimethylsiloxane)(PDMS)and poly(methyl meth-acrylate)(PMMA)and equipped with internal magnetic ministirrers and evaporation compensation by water replacement.In galactose-grown cells,the core genes of the GAL operon GAL2,GAL1,GAL7,and GAL10 were upregulated at least 100-fold relative to glucose-grown cells.These differential gene expression levels were similar to those observed in large-scale culture vessels.The increasing rate at which complete genomic sequences of microorganisms are becoming available offers an unprecedented opportunity for comparative investigations of these organisms.Our results from S.cerevisiae cultures grown in instrumented microbioreactors show that it is possible to integrate high-throughput studies of growth physiology with global gene expression analysis of microorganisms.
机译：将实时生长动力学测量与微生物培养物的整体基因表达分析相结合对于高通量生物学研究具有重要意义。我们已经在150μL生物反应器中的半乳糖和葡萄糖培养基中生长的真核模型酿酒酵母中进行了差异基因表达分析。配备用于实时和实时测量光密度（OD），pH和溶解氧（DO）的传感器。微生物反应器由聚二甲基硅氧烷（PDMS）和聚甲基丙烯酸甲酯（PMMA）制成在半乳糖生长的细胞中，GAL操纵子GAL2，GAL1，GAL7和GAL10的核心基因相对于葡萄糖生长的细胞上调了至少100倍。基因表达水平与在大型培养皿中观察到的水平相似。完整的微生物基因组序列逐渐增加ailable为这些生物的比较研究提供了前所未有的机会。我们在仪器化微生物反应器中生长的酿酒酵母培养物的结果表明，可以将生长生理学的高通量研究与微生物的全球基因表达分析相结合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号