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首页> 外文期刊>Biotechnology Progress >Design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: Optimization and synergism studies
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Design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: Optimization and synergism studies

机译：用于有效水解甘蔗渣的由不同真菌平台组成的酶混合物的设计：优化和协同研究

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Lignocellulosic materials represent a very important and promising source of renewable biomass. In order to turn them into fermentable sugars, synergism among the different enzymes that carry out bioconversion of these materials is one of the main factors that should be considered. Experimental mixture design was performed to optimize the proportion of enzymes produced by native strains of Trichoderma harzianum IOC 3844, Penicillium funiculosum ATCC 11797, and Aspergillus niger ATCC 1004, resulting in a proportion of 15, 50, and 35%, respectively. This mixture was able to hydrolyze 25 g/L of pretreated sugarcane bagasse with 91% of yield after 48 h of enzymatic reaction. Synergism along the hydrolysis process, besides the influence of lignin, hemicellulose, and solids loading, were also studied. Response surface methodology (RSM) based on Central Composite Rotatable Design was used to optimize solids and protein loadings to increase glucose release and enzymatic hydrolysis yield. The optimum solid and protein loadings established with RSM were 196 g/L and 24 mg/g cellulose, respectively, and under these conditions (94.1 +/- 8) g/L of glucose were obtained, corresponding to a hydrolysis yield of 64%. (c) 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1222-1229, 2016
机译：木质纤维素材料是可再生生物质的非常重要和有希望的来源。为了将它们变成可发酵的糖，进行这些材料的生物转化的不同酶之间的协同作用是应考虑的主要因素之一。进行实验混合物设计以优化由哈茨木霉IOC 3844，真菌青霉ATCC 11797和黑曲霉ATCC 1004的天然菌株产生的酶的比例，分别达到15、50和35％的比例。酶促反应48小时后，该混合物能够水解91 g / L预处理的甘蔗渣，收率为91％。除了木质素，半纤维素和固体含量的影响外，还研究了水解过程中的协同作用。基于中央复合旋转设计的响应表面方法（RSM）用于优化固体和蛋白质负载，以增加葡萄糖的释放和酶促水解的产量。用RSM确定的最佳固体和蛋白质负载量分别为196 g / L和24 mg / g纤维素，在这些条件下（94.1 +/- 8）获得了g / L的葡萄糖，相当于64％的水解率。 （c）2016美国化学工程师学会生物技术学会。 Prog。，32：1222-1229，2016

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