...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Alcohol >Chronic alcoholism alters the transport characteristics of folate in rat renal brush border membrane.
【24h】

Chronic alcoholism alters the transport characteristics of folate in rat renal brush border membrane.

机译：慢性酒精中毒会改变叶酸在大鼠肾刷边界膜中的转运特性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Folic acid transport across the epithelial cell membrane of kidney tubules is an essential step for its reabsorption, conservation, and homeostasis in the body. We characterized [(3)H]-folic acid transport in renal brush border membrane vesicles after 12 weeks of chronic ethanol ingestion to rats. The results demonstrated that chronic ethanol administration decreased the renal tubular reabsorption by a mechanism which involved an increase in the value of K(m) and a decrease in V(max). Importantly, ethanol feeding also interfered with disulfide bond status, temperature sensitivity, and Na(+) and divalent cation dependency of the transport process. The transport was transmembrane pH dependent, and ethanol did not have any effect on the pH optimum of the folate transport. The reduction in uptake in the ethanol-fed group was more pronounced at pH less than 6. In addition, the binding component was found to contribute to an appreciable extent to the total folate uptake; however, the amount of folate bindingwas less in the ethanol-fed group. Moreover, the folic acid analog methotrexate inhibited the transport to great degrees in control and ethanol-fed rats. These findings highlight the possible mechanism of renal disturbances of folate conservation during chronic alcoholism.
机译：叶酸跨肾小管的上皮细胞膜运输是其在体内重吸收，保守和体内平衡的必不可少的步骤。我们表征了[（3）H]-叶酸在慢性乙醇摄入大鼠12周后在肾刷缘膜囊泡中的转运。结果表明，长期服用乙醇可以通过增加K（m）值和降低V（max）的机制降低肾小管的重吸收。重要的是，乙醇进料还干扰了二硫键的状态，温度敏感性以及Na（+）和运输过程中二价阳离子的依赖性。该转运是跨膜pH依赖性的，并且乙醇对叶酸转运的最适pH没有任何影响。在pH低于6的情况下，以乙醇喂养的组的摄入量减少更为明显。此外，发现结合成分对总叶酸摄入量有可观的贡献。但是，乙醇喂养组的叶酸结合量较少。此外，叶酸类似物甲氨蝶呤在对照和乙醇喂养的大鼠中极大地抑制了转运。这些发现突出了慢性酒精中毒期间肾脏对叶酸保守性的干扰的可能机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号