Effect of ethyleneoxide groups of anionic surfactants on lipase activity

Magalhaes Solange S.; Alves Luis; Sebastiao Marco; Medronho Bruno; Almeida Zaida L.; Faria Tiago Q.; Brito Rui M. M.; Moreno Maria J.; Antunes Filipe E.

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Effect of ethyleneoxide groups of anionic surfactants on lipase activity

机译：阴离子表面活性剂的环氧乙烷基团对脂肪酶活性的影响

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The use of enzymes in laundry and dish detergent products is growing. Such tendency implies dedicated studies to understand surfactant-enzyme interactions. The interactions between surfactants and enzymes and their impact on the catalytic efficiency represent a central problem and were here evaluated using circular dichroism, dynamic light scattering, and enzyme activity determinations. This work focuses on this key issue by evaluating the role of the ethyleneoxide (EO) groups of anionic surfactants on the structure and activity of a commercial lipase, and by focusing on the protein/surfactant interactions at a molecular level. The conformational changes and enzymatic activity of the protein were evaluated in the presence of sodium dodecyl sulfate (SDS also denoted as SLE0S) and of sodium lauryl ether sulfate with two EO units (SLE2S). The results strongly suggest that the presence of EO units in the surfactant polar headgroup determines the stability and the activity of the enzyme. While SDS promotes enzyme denaturation and consequent loss of activity, SLE2S preserves the enzyme structure and activity. The data further highlights that the electrostatic interactions among the protein groups are changed by the presence of the adsorbed anionic surfactants being such absorption mainly driven by hydrophobic interactions. (c) 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1276-1282, 2016

机译：洗衣和餐具洗涤剂产品中酶的使用正在增长。这种趋势意味着需要专门研究来了解表面活性剂-酶的相互作用。表面活性剂和酶之间的相互作用及其对催化效率的影响是一个中心问题，在这里使用圆二色性，动态光散射和酶活性测定进行了评估。这项工作通过评估阴离子表面活性剂的环氧乙烷（EO）基团对商业脂肪酶的结构和活性的作用，以及在分子水平上关注蛋白质/表面活性剂的相互作用，着重解决了这一关键问题。在十二烷基硫酸钠（SDS也称为SLE0S）和具有两个EO单元的月桂基醚硫酸钠（SLE2S）的存在下评估蛋白质的构象变化和酶活性。结果强烈表明表面活性剂极性头基中EO单元的存在决定了酶的稳定性和活性。 SDS促进酶变性并因此失去活性，而SLE2S保留酶的结构和活性。数据进一步突显了蛋白质基团之间的静电相互作用被吸附的阴离子表面活性剂的存在所改变，所述阴离子表面活性剂主要是由疏水相互作用驱动的。（c）2016美国化学工程师学会生物技术学会。 Prog。，32：1276-1282，2016

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《Biotechnology Progress》 |2016年第5期|共7页
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Magalhaes Solange S.; Alves Luis; Sebastiao Marco; Medronho Bruno; Almeida Zaida L.; Faria Tiago Q.; Brito Rui M. M.; Moreno Maria J.; Antunes Filipe E.;
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正文语种 eng
中图分类生物科学;
关键词
surfactant; enzyme interaction; enzymatic activity; denaturation; hydrophobic association; ethyleneoxide;

机译：表面活性剂;酶相互作用;酶活性;变性;疏水缔合;环氧乙烷;

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1. Effect of ethyleneoxide groups of anionic surfactants on lipase activity [J] . Magalhaes Solange S., Alves Luis, Sebastiao Marco, Biotechnology Progress . 2016,第5期

机译：阴离子表面活性剂的环氧乙烷基团对脂肪酶活性的影响
2. Titrimetric determination of anionic surfactant content in anioniconionic surfactant mixture solution by anionic surfactant selective electrode [J] . Junwei WANG, Zhiping DU, Wanxu WANG Turkish journal of chemistry . 2012,第4期

机译：阴离子表面活性剂选择电极滴定法测定阴离子/非离子表面活性剂混合溶液中阴离子表面活性剂的含量
3. Titrimetric determination of anionic surfactant content in anioniconionic surfactant mixture solution by anionic surfactant selective electrode [J] . JUNWEI WANG, ZHIPING DU, WANXU WANG, Turkish journal of chemistry . 2012,第4期

机译：阴离子表面活性剂选择电极滴定法测定阴离子/非离子表面活性剂混合溶液中阴离子表面活性剂的含量
4. Synergistic Effects of Anionic Surfactant and 0.30 T of Static Magnetic Field on the Activity and Structure of Catalase [C] . Jingyu Ran, Shaoyi Jia, Yong Liu, AIChE annual meeting . 2010

机译：阴离子表面活性剂和0.30 T静磁场对过氧化氢酶活性和结构的协同作用
5. STUDIES ON THE USE OF SURFACTANT SYSTEMS IN ANALYTICAL CHEMISTRY. PART I. THE DETERMINATION OF THE DISSOCIATION CONSTANTS OF OXINE AND 2-METHYLOXINE IN THE PRESENCE OF MICELLE-FORMING SURFACTANTS. PART II. THE DETERMINATION OF SOME OF METAL IONS WITH OXINE AND 2-METHYLOXINE IN ANIONIC, CATIONIC AND NON-IONIC SURFACTANTS. PART III. THE DETERMINATION OF NITRITE WITH 2-METHYLOXINE IN CATIONIC SURFACTANT. PART IV. THE DETERMINATION OF METAL IONS WITH. [D] . TSAO, FU-PAO. 1981

机译：表面活性剂系统在分析化学中的应用研究。第一部分。形成胶束的表面活性剂存在下氧和2-甲基氧肟的离解常数的测定。第二部分阴离子表面活性剂，阳离子表面活性剂和非离子表面活性剂中一些金属离子的含量测定，分别采用氧化和2-甲氧基。第三部分阳离子表面活性剂中2-甲基氧肟酸的亚硝酸根的测定第四部分金属离子的测定。
6. EFFECT OF INORGANIC CATIONS ON BACTERICIDAL ACTIVITY OF ANIONIC SURFACTANTS [O] . J. G. Voss 1963

机译：无机阳离子对阴离子表面活性剂杀菌活性的影响
7. Penetration of Anionic Surfactants into Skin II. Study of Mechanisms Which Impede the Penetration of Synthetic Anionic Surfactants into Skin11From the Research Laboratories of the Department of Dermatology of the Harvard Medical School at the Massachusetts General Hospital, Boston 14, Massachusetts.This investigation was supported by grants from the U. S. Army Chemical Corps (Contract DA 18-108-405-CML-672) and from the Division of Medical Sciences of the Department of Health, Education and Welfare, U. S. Public Health Service, National Institutes of Health (Grant RG-4760).We wish to thank Colgate-Palmolive Co., Lever Brothers Co., The Procter and Gamble Co., Monsanto Chemical Co., and California Research Corporation for the various surfactants used in this investigation. Mr. Roy W. Peet, Manager of the Association of American Soap and Glycerine Producers, Inc., helped us make contact with the surfactant manufacturers. [O] . Blank I.H., Gould E. 1961

机译：阴离子表面活性剂渗透到皮肤中II。阻碍合成阴离子表面活性剂渗透到皮肤中的机理的研究11来自马萨诸塞州波士顿市麻萨诸塞州波士顿市麻省总医院哈佛医学院皮肤科的研究实验室。该研究得到了美国陆军化学总队的资助（合同DA 18-108-405-CML-672）和美国国立卫生研究院公共卫生服务局卫生，教育与福利部医学科（Grant RG-4760），我们要感谢高露洁-Palmolive公司，Lever兄弟公司，Procter and Gamble公司，Monsanto化学公司和California Research Corporation，用于本次调查中使用的各种表面活性剂。美国肥皂和甘油生产商协会的经理Roy W. Peet先生帮助我们与表面活性剂制造商联系。

Effect of ethyleneoxide groups of anionic surfactants on lipase activity

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