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Sugar recovery and fermentability of hemicellulose hydrolysates fromsteam-exploded softwoods containing bark

机译:从含有树皮的蒸汽炸软木中回收半纤维素水解物的糖分和发酵性

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The hemicellulose sugar recovery and ethanol production obtained from SO2-catalyzed steam explosion of a mixed white fir (70%) and ponderosa pine (30%) feedstock containing bark (9% dry weight/dry weight) was assessed. More than 90% of the available hemicellulose sugars could be recovered in the hydrolysate obtained after steam explosion at 195 degreesC, 2.38 min, and 3.91% SO2, with 59% of the original hemicellulose sugars detected in a monomeric form. Despite this high sugar recovery, this hydrolysate showed low ethanol yield (64% of theoretical yield) when fermented with a spent sulfite liquor-adapted strain of Saccharomyces cerevisiae. In contrast, most hydrolysates prepared at higher steam explosion severity showed comparable or higher ethanol yields. Furthermore, the hydrolysates prepared from bark-free feedstock showed better fermentability (87% of theoretical yield) despite containing higher concentration of known inhibitors. The ethanol yield from the hydrolysate prepared from a bark-containing wood sample could be improved to 81% by an extra stage acid hydrolysis (121 degreesC for 1 h in 3% sulfuric acid). This extra stage acid hydrolysis and steam explosion at higher severity conditions seem to improve the fermentability of the hydrolysates by transforming certain inhibitory compounds present in the hydrolysates prepared from the bark-containing feedstock and thus lowering their inhibitory effect on the yeast used for the ethanol fermentation.
机译:评估了由SO2催化的蒸汽爆炸(混合白杉(70%)和美国黄松(30%)含树皮(干重9%/干重))得到的半纤维素糖回收率和乙醇产量。在195℃,2.38分钟和3.91%的SO2蒸汽爆炸后获得的水解物中,可以回收到90%以上的可用半纤维素糖,其中59%的原始半纤维素糖以单体形式被检测到。尽管有很高的糖回收率,但是当该水解产物用废亚硫酸盐酒改种的酿酒酵母菌株发酵时,乙醇收率仍然很低(理论收率的64%)。相反,大多数在较高的蒸汽爆炸强度下制备的水解产物显示出相当或更高的乙醇产率。此外,由无树皮原​​料制得的水解产物尽管含有较高浓度的已知抑制剂,但仍显示出更好的发酵性(理论收率的87%)。通过含树皮的木材样品制备的水解产物,乙醇的收率可以通过额外阶段的酸水解(在3%硫酸中于121℃下放置1小时)提高到81%。在较高苛刻条件下进行的这种额外的酸水解和蒸汽爆炸似乎通过转化存在于由含树皮的原料制得的水解物中的某些抑制化合物,从而改善了水解产物的发酵能力,从而降低了其对用于乙醇发酵的酵母的抑制作用。

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