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首页> 外文期刊>AJRI: American Journal of Reproductive Immunology >Type-1 and type-2 cytokines in human decidual tissue and trophoblasts from normal and abnormal pregnancies detected by reverse transcriptase polymerase chain reaction (RT-PCR).
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Type-1 and type-2 cytokines in human decidual tissue and trophoblasts from normal and abnormal pregnancies detected by reverse transcriptase polymerase chain reaction (RT-PCR).

机译：通过逆转录聚合酶链反应（RT-PCR）检测到正常和异常妊娠的人蜕膜组织和滋养细胞中的1型和2型细胞因子。

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PROBLEM: Cytokine expression at the maternal fetal interface has been well documented in rodents, but data in the human are scanty and controversial. METHOD OF STUDY: We examined cytokine expression of human decidua and trophoblasts by semiquantitative visual grading of reverse transcriptase polymerase chain reaction (RT-PCR) products in five groups of patients: ten patients with uncomplicated term pregnancies undergoing elective cesarean section (Group 1); ten women having normal pregnancies at term and vaginal delivery (Group 2); ten patients having intrauterine growth-retarded infants of unknown cause after a spontaneous vaginal delivery at term (Group 3); ten childless women having their first, first-trimester spontaneous abortion (Group 4); and ten childless women with a history of one or more previous first-trimester spontaneous abortions and having a new miscarriage (Group 5). RESULTS: Results favoring the T-helper 1 (Th1)/T-helper 2 (Th2) model during pregnancy were: significantly higher expression of interferon gamma (IFN-gamma) in trophoblast samples from Group 3 versus 2 and in decidual tissue from Group 5 versus 4; stronger positivity of interleukin (IL)-10 in decidual tissue samples from Group 1 versus Groups 2 and 5; and higher expression levels of tumor necrosis factor-beta (TNF)-beta by the trophoblast in Group 5 versus 1. Against the Th1/Th2 paradigm were the following findings: the significantly increased expression of IFN-gamma by decidual or trophoblast samples in Groups 1 versus 2, 2 versus 3, and 1 versus 5; and the significantly higher expression of TNF-alpha in decidual samples from patients in Group 1 (but also Group 4) as compared with Group 5. IL-2 mRNA and IL-4 mRNA could not be detected. CONCLUSIONS: Overall, our findings suggest that there is a balance between type-1 and type-2 cytokines during pregnancy, which is mainly characterized by the expression of IFN-gamma (a type-1 cytokine) and IL-10 (a type-2 cytokine) at the maternal fetal interface.
机译：问题：在啮齿动物中，母体胎儿界面上的细胞因子表达已有很好的文献记载，但人类的数据很少且有争议。研究方法：我们通过逆转录酶聚合酶链反应（RT-PCR）产品的半定量视觉分级，检查了五组患者的人蜕膜和滋养细胞的细胞因子表达：十例行择期剖宫产的未妊娠足月患者（第一组）;十名足月和阴道分娩正常的孕妇（第二组）;足月自发阴道分娩后，有十名患者因子宫内发育迟缓而原因不明的婴儿（第3组）;十名无子女的妇女在初，初三个月自然流产（第4组）;十名无子女的妇女，有一个或多个以前的上三个月自然流产的历史，并且有新的流产（第5组）。结果：怀孕期间支持T-helper 1（Th1）/ T-helper 2（Th2）模型的结果是：第3组滋养细胞样品中干扰素γ（IFN-γ）的表达明显高于第2组和蜕膜组织中的干扰素。 5比4;第1组与第2组和第5组相比，蜕膜组织样品中白介素（IL）-10的阳性率更高;与第1组相比，第5组的滋养细胞具有更高的肿瘤坏死因子-β（TNF）-β表达水平。针对Th1 / Th2范式有以下发现：蜕膜或滋养细胞样品中的IFN-γ表达显着增加1对2、2对3和1对5;与第5组相比，第1组（以及第4组）患者蜕膜中TNF-α的表达明显更高。无法检测到IL-2 mRNA和IL-4 mRNA。结论：总的来说，我们的发现表明，怀孕期间1型和2型细胞因子之间存在平衡，其主要特征在于IFN-γ（1型细胞因子）和IL-10（2型细胞因子）的表达。 2细胞因子）在孕妇的胎儿界面。

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