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首页> 外文期刊>DNA and Cell Biology >Associations of Genetic Polymorphisms Relevant to Metabolic Pathway of Vitamin D3 with Development and Prognosis of Childhood Bronchial Asthma
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Associations of Genetic Polymorphisms Relevant to Metabolic Pathway of Vitamin D3 with Development and Prognosis of Childhood Bronchial Asthma

机译：遗传多态性与儿童支气管哮喘发育及预后的维生素D3相关遗传多态性相关性

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摘要

This study was aimed at investigating the correlation between genetic polymorphisms relevant to metabolic pathway of vitamin D3 (VD3) and susceptibility to childhood bronchial asthma. Altogether 143 childhood patients with bronchial asthma and 143 healthy children of Chinese Han ethnicity were enrolled in this study. The key single- nucleotide polymorphisms (SNPs) were identified by HaploView 4.2 software and selected from previous investigations. Genomic DNAs were isolated from peripheral blood samples by using TaqMan Blood DNA kits. The genotyping of SNPs was performed by TaqMan SNPs genotyping assay. Odds ratios and corresponding 95% confidence intervals were calculated to evaluate the association between SNPs and susceptibility to bronchial asthma. Statistical analyses were conducted by using SPSS 13.0 software. Rs10766197 of CYP2R1, rs7041 and rs4588 of CG, rs4646536 of CYP27B1, rs2228570, rs7975232, and rs1544410 of VDR, as well as rs1805192 and rs10865710 of PPAR were shown to be significantly associated with increased risk of bronchial asthma. Besides, prognosis of childhood bronchial asthma, which was represented as Saint George Respiratory Questionnaire (SGRQ) scoring, was closely linked with CYP2R1 rs10766197, CYP27B1 rs4646536, VDR rs7975232, VDR rs1544410, PPAR rs1805192, and PPAR rs10865710. The haplotype analysis suggested that TA and CG of CG rs7041/rs4588, CA and AG of VDR rs7975232/rs1544410, and CC of PPAR rs1805192/rs10865710 were, respectively, correlated with levels of VD, IL- 4, and IL- 5. And only haplotypes of VDR showed associations with risk of bronchial asthma during childhood, whereas hardly any significance could be observed between the haplotypes and behavior of quality- of- life (SGRQ) scoring. Significant associations were found between rs10766197 of CYP2R1, rs7041 and rs4588 of CG, rs4646536 of CYP27B1, rs2228570, rs7975232, and rs1544410 of VDR, as well as rs1805192 and rs10865710 of PPAR and susceptibility to and prognosis of childhood bronchial asthma, providing novel targets for treating the disorder.
机译：本研究旨在研究与维生素D3（VD3）代谢途径相关的遗传多态性与儿童支气管哮喘的易感性相关。共有143名儿童患有支气管哮喘和143名中国汉族的健康儿童的儿童患者。通过Haploview 4.2软件鉴定关键的单核苷酸多态性（SNPs），并选自先前的研究。通过使用Taqman血液DNA试剂盒从外周血样品中分离基因组DNA。通过Taqman SNPS基因分型测定进行SNP的基因分型。计算差距和相应的95％置信区间以评价SNP与支气管哮喘的易感性之间的关联。使用SPSS 13.0软件进行统计分析。 CYP2R1的Rs10766197，rs7041和CG，CYP27B1，rs2228570，rs7975232，和VDR的rs1544410的rs4646536，以及rs1805192和PPAR的rs10865710的rs4588显示出支气管哮喘的风险增加相关联显著。此外，被称为Saint George呼吸问卷（SGRQ）评分的儿童支气管哮喘的预后与CYP2R1 RS10766197，CYP27B1 RS4646536，VDR RS7975232，VDR RS154410，PPAR RS1805192和PPAR RS10865710密切相关。单倍型分析表明，VDR RS7975232 / RS154410的CG RS7041 / RS4588，CA和AG的TA和CG和PPAR RS1805192 / RS10865710的CC分别与VD，IL-4和IL-5的水平相关。只有VDR的单倍型显示童年时期的带支气管哮喘风险的关联，而几乎没有任何重要性，在生活质量（SGRQ）评分的单倍型和行为之间可以观察到。 CG，RS466536的CYP2R1，RS7041和RS4588的CY10766197在CYP27B1，RS22228570，RS7975232和VDR的RS1805192的RS1805192和儿童支气管哮喘的易感性Rs1805192和Rs10865710之间进行了重要关联，提供了新的靶标治疗这种疾病。

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