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首页> 外文期刊>Developmental and Comparative Immunology: Ontogeny, Phylogeny, Aging: The Official Journal of the International Society of Developmental and Comparative Immunology >20-Hydroxyecdysone regulates the transcription of the lysozyme via Broad-Complex Z2 gene in silkworm, Bombyx mori
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20-Hydroxyecdysone regulates the transcription of the lysozyme via Broad-Complex Z2 gene in silkworm, Bombyx mori

机译：20-羟基迪克松通过蚕丝，BOMBYX MORI通过广泛的综合Z2基因调节溶菌酶的转录

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Broad-Complex Z2 (Br-C Z2) is an ecdysone inducible transcription factor that regulates physiological, innate immune and developmental events in insects. Here, we identified an orthologue of Br-C Z2 from silkworm, Bombyx mori (BmBr-C Z2) to study its involvement in immune responses. The quantitative real-time PCR analysis revealed that BmBr-C Z2 was expressed ubiquitously in all tested tissues under normal physiological conditions. Further, developmental profile displayed that BmBr-C Z2 expression was detectable in different developmental stages, however the genes expression was highest in the molting and pre-pupal stages. Administration of 20hydroxyecdysone (20E) enhanced the expression levels of BmBr-C Z2 in hemocytes. The challenge with pathogens and pathogen associated molecular patterns (PAMPs) also upregulated the mRNA levels of BmBr-C Z2 in hemocytes when compared with the control. By contrast, the ectopic expression of BmBr-C Z2 remarkably increased the production of antimicrobial peptides, while the knock-down of this gene by double stranded RNA decreased their production. Dual-luciferase assay exhibited that BmBr-C Z2 induced the expression of lysozyme by directly binding to its promoter region. The treatment of Escherichia coli following the knock-down of BrnBr-C Z2 strongly reduced the survival rate of silkworm larvae. These results suggest that BmBr-C Z2 plays an important biological role in the innate immune responses of silkworm by regulating immune-related genes.
机译：宽复合Z2（BR-C Z2）是一种蜕皮型诱导转录因子，调节昆虫中的生理，先天免疫和发育事件。在这里，我们鉴定了来自蚕，Bombyx Mori（BMBR-C Z2）的BR-C Z2的正门，以研究其参与免疫应答。定量实时PCR分析显示，在正常生理条件下，BMBr-C Z2在所有测试组织中普遍地表达。此外，在不同的发育阶段中可以检测到BMBr-C Z2表达的发育轮廓，但是在蜕皮和蛹前阶段中的基因表达是最高的。施用20个羟基倍晶酮（20e）增强了血细胞中BMBR-C Z2的表达水平。与对照相比，病原体和病原体相关分子模式（PAMPs）的挑战还上调了血细胞中BMBR-C Z2的mRNA水平。相比之下，BMBr-C Z2的异位表达显着增加了抗微生物肽的产生，而这种基因的双链RNA的敲低度降低了它们的生产。双荧光素酶测定结果表明，BMBR-C Z2通过直接结合其启动子区诱导溶菌酶的表达。 BRNBR-C Z2倒闭后大肠杆菌的治疗强烈降低了蚕幼虫的存活率。这些结果表明，BMBR-C Z2通过调节免疫相关基因在家蚕的先天免疫应答中起着重要的生物学作用。

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