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首页> 外文期刊>Developmental and Comparative Immunology: Ontogeny, Phylogeny, Aging: The Official Journal of the International Society of Developmental and Comparative Immunology >Identification and characterization of an atypical RIG-I encoded by planarian Dugesia japonica and its essential role in the immune response
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Identification and characterization of an atypical RIG-I encoded by planarian Dugesia japonica and its essential role in the immune response

机译：由Planian Dugesia japonica编码的非典型钻井平台的鉴定与表征及其在免疫应答中的基本作用

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Retinoic acid-inducible gene I (RIG-I), an RNA sensor with a conserved structure, activates the host interferon (IFN) system to produce IFNs and cytokines for eliminating pathogens upon recognizing PAMPs. However, the biological functions and the mechanism by which RIG-I regulates the innate immunity response in invertebrates are still unknown at present. Here we identified an atypical RIG-I in planarian Dugesia japonica. Sequence analysis, 3D structure modeling and phylogenetic analysis showed that this atypical protein was clustered into a single Glade at the base of the tree in invertebrates, suggesting that DjRIG-I is an ancient and unique protein of the RIG-I-like receptors (RLRs). In situ hybridization analysis revealed that the DjRIG-I mRNAs were predominantly expressed in the pharynx and head of the adult and regenerative planarians. Stimulation with PAMPs induced the over-expression of DjRIG-I in planarians. The molecular simulation demonstrated that DiRIG-I formed a large hole-structure for the docking of dsRNAs, and the pull-down assay confirmed the interaction between DIRIG-I and viral analog poly(I:C). Importantly, some representative antiviral/antibacterial genes in the RIG-I-mediated IFN and P38 signaling pathway, TBK1, IRF-3, Mx, and P38, were significantly upregulated in planarians stimulated with PAMPs. Interference of the DjRIG-I expression by RNAi, inhibited the PAMPs-induced over-expression, suggesting that DjRIG-I is a key player for downstream signaling events. These results indicate that DjRIG-I triggered the intracellular signaling cascades independent of the classical CARD domains and played an essential role in the virus/bacteria-induced innate immunity of planarian.
机译：视黄酸诱导基因I（RIG-I），具有保守结构的RNA传感器，激活宿主干扰素（IFN）系统，以产生IFNS和细胞因子，用于在识别泵时消除病原体。然而，目前仍然未知的生物功能和钻井钻井液中的机制仍然不为人知。在这里，我们在Planirarairdugesia japonica确定了一个非典型钻机。序列分析，3D结构建模和系统发育分析表明，这种非典型蛋白质在无脊椎动物的树底部聚集成单个林位，表明Djrig-i是钻机-i般受体的古老而独特的蛋白质（RLRS ）。原位杂交分析表明，Djrig-1 mRNA主要在成人和再生平面图的咽部和头部主导地表达。用PAMP刺激诱导平面图中Djrig-i的过表达。分子模拟表明Dirig-i形成了用于对接的大孔结构，用于对接的DSRNA，下拉测定证实了DIRIG-I和病毒性模拟聚（I：C）之间的相互作用。重要的是，在用PAMP刺激的平面图中显着上调，在钻井型IFN和P38信号传导途径，TBK1，IRF-3，MX和P38中的一些代表性抗病毒/抗菌基因，TBK1，IRF-3，MX和P38显着上调。 RNAi的DJRIG-I表达的干扰抑制了PAMP诱导的过表达式，表明DJRIG-i是下游信令事件的关键球员。这些结果表明，Djrig-i触发了独立于古典卡片结构域的细胞内信号传导级联，并在病毒/细菌诱导的平面图中发挥了重要作用。

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