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首页> 外文期刊>Dyes and Pigments >Development of a novel near-infrared fluorescence light-up probe with a large Stokes shift for sensing of cysteine in aqueous solution, living cells and zebrafish
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Development of a novel near-infrared fluorescence light-up probe with a large Stokes shift for sensing of cysteine in aqueous solution, living cells and zebrafish

机译：开发一种新型近红外荧光升降探针，具有大型斯托克斯的速度，用于感应水溶液中半胱氨酸，活细胞和斑马鱼

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摘要

As an important sulfur-containing amino acid, aberrant levels of cysteine are closely related with an array of dieases. Although many Cys-specific fluorescent probes have been designed, most of them wouldn't be able to detect Cys in buffer solution, which limits the application of the probe in biological system. In this work, a novel near-infrared fluorescent probe Cys-WR with a large Stokes shift (about 110 nm) was developed for the detection of Cys by conjugating the fluorophore WR-4 with crotonate moiety. The probe exhibited off-on response to Cys with wide linear range of 0-100 mu M, as well as the color of the probe solution changes from deep pink to brown observed by naked eyes. Moreover, the probe showed little cytotoxicity. Imaging of Cys using this probe in living A549 cells and zebrafish had also been well demonstrated.
机译：作为一种重要的含硫氨基酸，多种半胱氨酸水平与脱脂阵列密切相关。 尽管设计了许多CYS特异性荧光探针，但大多数将无法检测缓冲溶液中的CYS，这限制了探针在生物系统中的应用。 在这项工作中，通过将荧光团WR-4与裆部部分缀合，开发出具有大型斯托克斯偏移（约110nm）的新型近红外荧光探针Cys-WR。 探针对具有0-100 mu m的宽线性范围的Cys表现出偏离响应，以及探针溶液的颜色从肉眼观察到的深粉红色到褐色。 此外，探针显示出很少的细胞毒性。 在生活A549细胞和斑马鱼中使用这种探针的Cys成像也得到了很好的证明。

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  • 来源
    《Dyes and Pigments》 |2019年第2019期|共5页
  • 作者

    Zhang Xiaoyu; Liu Heng; Ma Yingying; Qu Wangbo; He Hanping; Zhang Xiuhua; Wang Shengfu; Sun Qi; Yu Fabiao;

  • 作者单位

    Hubei Univ Minist Educ Key Lab Synth &

    Applicat Organ Funct Mol Hubei Collaborat Innovat Ctr Adv Organ Chem Mat Wuhan 430062 Hubei Peoples R China;

    Hubei Univ Minist Educ Key Lab Synth &

    Applicat Organ Funct Mol Hubei Collaborat Innovat Ctr Adv Organ Chem Mat Wuhan 430062 Hubei Peoples R China;

    Hubei Univ Minist Educ Key Lab Synth &

    Applicat Organ Funct Mol Hubei Collaborat Innovat Ctr Adv Organ Chem Mat Wuhan 430062 Hubei Peoples R China;

    Hubei Univ Minist Educ Key Lab Synth &

    Applicat Organ Funct Mol Hubei Collaborat Innovat Ctr Adv Organ Chem Mat Wuhan 430062 Hubei Peoples R China;

    Hubei Univ Minist Educ Key Lab Synth &

    Applicat Organ Funct Mol Hubei Collaborat Innovat Ctr Adv Organ Chem Mat Wuhan 430062 Hubei Peoples R China;

    Hubei Univ Minist Educ Key Lab Synth &

    Applicat Organ Funct Mol Hubei Collaborat Innovat Ctr Adv Organ Chem Mat Wuhan 430062 Hubei Peoples R China;

    Hubei Univ Minist Educ Key Lab Synth &

    Applicat Organ Funct Mol Hubei Collaborat Innovat Ctr Adv Organ Chem Mat Wuhan 430062 Hubei Peoples R China;

    Wuhan Inst Technol Minist Educ Key Lab Green Chem Proc Wuhan 430205 Hubei Peoples R China;

    Hainan Med Univ Key Lab Hainan Trauma &

    Disaster Rescue Key Lab Emergency &

    Trauma Inst Funct Mat &

    Mol I Coll Emergency &

    Trauma Coll Clin Med Minist Educ Haikou 571199 Hainan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 染料及中间体工业;颜料工业;
  • 关键词

    Fluorescent probes; Near-infrared emission; Cell imaging; In vivo imaging; Cysteine;

    机译：荧光探针;近红外发射;细胞成像;体内成像;半胱氨酸;

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