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Porous silicon-based fluorescent nanoprobe for the detection of anthrax biomarker and its practical sensing applications

机译：用于检测炭疽生物标志物的多孔硅基荧光纳米孔及其实用传感应用

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Anthrax is an infection caused by bacterium Bacillus anthracis (B. anthracis), and is an old disease that poses a new threat to humans as a biological weapon. Gram-staining and polymerase chain reaction (PCR)-based assays of specimens are standard diagnostic tools for anthrax, but it is time-consuming and limited for the on-site application. In this study, we disclose a porous silicon-based nanoprobe for the detection of dipicolinic acid (DPA), which is a crucial biomarker of the anthrax spore, and its practical sensing applications. The surface of the porous silicon nanoparticles (pSiNPs) was functionalized with multiple amine arms, via a ring-opening click amination and was grafted with terbium ion (Tb3+). A double antenna effect from DPA and pSiNPs to Tb3+ was appeared dramatically and was accompanied by a significant fluorescence enhancement that had high selectivity, sensitivity, and fast response time. The resulting pSiNPs-Tb formulation was used to detect DPA in real water samples and the stimulated Bacillus subtilis (B. subtilis) spores. Besides, the DPA sensing application on the cellulose-based paper was successfully demonstrated as a real-time on-site sensing kit. We believe the demonstrations in this study could encourage further applications throughout a wide variety of fields.
机译：炭疽是由杆菌杆菌（B.炭疽病）引起的感染，是一种造成对人类作为生物武器的新威胁的旧疾病。基于克染色和聚合酶链反应（PCR）的标本的测定是炭疽的标准诊断工具，但对于现场应用是耗时和限制的。在该研究中，我们公开了一种用于检测二辛溶胶（DPA）的多孔硅基纳米孔，这是炭疽孢子的关键生物标志物，以及其实际传感应用。多孔硅纳米颗粒（Psinps）的表面用多个胺臂官能化，通过环开键键入胺化并用铽离子（Tb3 +）接枝。从DPA和Psinps到Tb3 +的双天线效应显着显着，伴随着具有高选择性，灵敏度和快速响应时间的显着荧光增强。得到的psinps-tb配方用于检测真实水样中的DPA和刺激的芽孢杆菌（B.枯草芽孢杆菌）孢子。此外，成功地证明了基于纤维素的纸张上的DPA感测应用是实时的现场感测套件。我们相信本研究中的示威活动可以鼓励在整个各种领域的进一步应用程序。

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