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首页> 外文期刊>Biotechnology Progress >Production of rerovirus and adenovirus vectors for gene therapy:a comparative study using microcarrier and stationary cell culture
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Production of rerovirus and adenovirus vectors for gene therapy:a comparative study using microcarrier and stationary cell culture

机译：轮状病毒和腺病毒载体用于基因治疗的生产：使用微载体和固定细胞培养的比较研究

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In gene therapy,retrovirus and adenovirus vectors are extensively used as genedilivery vehicles and further large-scale processing of these viralvectros will be increasingly important.This study examined stationary and microcarrier cell culture systems with respect to the production of a retrovirus vector (encoding a monounit hammerhead ribozyme gene with anintron) and anadenovirus vector (encoding a reporter lacZ gene).Cytodex 1 and Cytodex 3 solid microcarriers were found to be able to provide good cell growth and high-titer vector production in suspension sultures.Porous microcarriers such as Cytopore 2 gave slightly lower but still efficient growth but produced significantly lower titers of retrovirus and adenovirus vector from the producer cells.The specific retrovirus producttion of adenovirus vector in 293 cells.Hydrodynamic shear forceson microcarrier-titer production yield for retrovirus vector but decreased for adenovirus vector.The cellula rproductivity was much more efficient for adenovirus vector produced in 293 cells as compared to the retrovirus vector produced in PA317-RCM1 cells.These findings canprovide further insight into the feasibility of applying microcarrier cellculture technology toproduce gene-therapy virus vectors.
机译：在基因治疗中，逆转录病毒和腺病毒载体被广泛用作基因传递载体，对这些病毒载体的进一步大规模处理将变得越来越重要。这项研究检查了固定和微载体细胞培养系统与逆转录病毒载体（编码一个单位）的产生锤头状核酶基因（带有antron）和腺病毒载体（编码报告基因lacZ基因）.Cytodex 1和Cytodex 3固体微载体能够在悬浮培养液中提供良好的细胞生长和高滴度载体生产。多孔微载体（如Cytopore 2）腺病毒载体在293细胞中的特异性逆转录病毒生产。逆转录病毒载体的微流变滴度生产产量，但腺病毒载体的产量下降。纤维素的生产力要高得多与在PA317-RCM1细胞中产生的逆转录病毒载体相比，在293细胞中产生的腺病毒载体的nt。这些发现可以为使用微载体细胞培养技术生产基因治疗病毒载体的可行性提供进一步的认识。

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