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首页> 外文期刊>Biotechnology Progress >Production of cycloisomaltooligosaccharides from dextran using enzyme immobilized in multilayers onto porous membranes
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Production of cycloisomaltooligosaccharides from dextran using enzyme immobilized in multilayers onto porous membranes

机译：使用固定在多孔膜上的多层酶从右旋糖酐生产环异麦芽低聚糖

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摘要

Anion-exchange porous holllow-fiber membranes with a thickness of about 1.2 mm and a pore size fo about 0.30 #mu#m were used as a supporting matrix toimmobilize cycloisomaltooligosaccharide glucanotrasferase 9CITase).CITase was immobilized to the membrane via anion-exchange adsorptionand by subsequent enzymatic crosslinking with tansglutaminase,the amount of whichranged form 3 to 110 mg per gram of the membrane.The degree of enzyme multilayer binding was equivalent to 0.3-9.8.Dextran,as the substrae,was convrted intoseven-to nine-dglucose-memberd cycloisomaltooligosacharides (CI-7,-8,and -9) at a maximumyield of 28% in weight at a space velocity of 10 per hour during the permeation of 2.0% (w/w) dextran solution across the CITase-immobilized porous hollow-fiber membrane.Theyield of CIs increased with increasing degree of CITase multilayering.
机译：用厚度约1.2 mm，孔径约0.30＃μm的阴离子交换多孔空心纤维膜固定环异麦芽低聚糖葡糖苷酸转移酶9CITase），将CITase通过阴离子交换吸附法固定在膜上。随后用丹宁酰胺酶进行酶交联，每克膜的交联量为3至110 mg。酶多层结合的程度相当于0.3-9.8。作为底物的右旋糖酐被转化为7至9个葡萄糖元。在2.0％（w / w）右旋糖酐溶液透过固定有CITase的多孔中空纤维的过程中，以每小时10的空速，最大重量产率为28％的环异麦芽糖（CI-7，-8和-9）纤维膜。CI的产量随CITase多层化程度的增加而增加。

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