Identification of affinity ligands for protein purification from synthetic chemical combinatorial libraries

Yan Wang; Tingyu Li

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Identification of affinity ligands for protein purification from synthetic chemical combinatorial libraries

机译：从合成化学组合文库中鉴定用于蛋白质纯化的亲和配体

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A method to screen combinatorial libraries for the development of selective ligands for protein affinity chromatographic purification is described.The method is based on the application of parallel combinatorial libraties,and it ahs several potential advantages.The screening procedure is simple and sraight forward,and it does not require the chemical derivatization of the target proteins or even that the target protein be pure.The experiment can also be designed to select binders that are less likely to cause protein denaturation.Feasibilit of this approachis demonstraed with a model study of the chromatographic purification of bovine albuminserum (BSA) and Avidin.

机译：描述了一种筛选组合文库以开发用于蛋白质亲和层析纯化的选择性配体的方法。该方法基于平行组合文库的应用，具有多个潜在优点。筛选过程简单易行，而且不需要目标蛋白质的化学衍生化，甚至不需要目标蛋白质是纯净的。该实验还可以设计为选择不太可能引起蛋白质变性的结合剂。该方法的可行性通过色谱纯化的模型研究证明牛白蛋白（BSA）和抗生物素蛋白。

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《Biotechnology Progress》 |2002年第3期|共6页
作者
Yan Wang; Tingyu Li;
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正文语种 eng
中图分类生物工程学（生物技术）;分子生物学;
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1. Identification of affinity ligands for protein purification from synthetic chemical combinatorial libraries [J] . Yan Wang, Tingyu Li Biotechnology Progress . 2002,第3期

机译：从合成化学组合文库中鉴定用于蛋白质纯化的亲和配体
2. Utilizing a library of synthetic affinity ligands for the enrichment, depletion and one-step purification of leech proteins. [J] . Dong D, Gui Y, Chen D, Journal of molecular recognition: JMR . 2008,第3期

机译：利用合成的亲和配体库对水ech蛋白进行富集，耗竭和一步纯化。
3. The identification of high-affinity G protein-coupled receptor ligands from large combinatorial libraries using multicolor quantum dot-labeled cell-based screening [J] . FuJ., LeeT., QiX. Future medicinal chemistry . 2014,第7期

机译：使用多色量子点标记的基于细胞的筛查从大型组合文库中鉴定高亲和力G蛋白偶联受体配体
4. Development of a small molecule peptidomimetic affinity Ligand for efficient purification of the large protein Factor VIII [C] . Sebastian Knor, Burkhardt Laufer, Alexey Khrenov American Peptide Symposium . 2009

机译：小分子肽瘤亲和力配体的发展，用于有效纯化大蛋白质因子VIII
5. Purification of alpha -1 -proteinase inhibitor using ligands from combinatorial peptide libraries [D] . Bastek, Patrick David 2000

机译：使用组合肽库中的配体纯化α-1-蛋白酶抑制剂
6. The identification of high-affinity G protein-coupled receptor ligands from large combinatorial libraries using multicolor quantum dot-labeled cell-based screening [O] . Junjie Fu, Timothy Lee, Xin Qi -1

机译：使用多色量子点标记的基于细胞的筛查从大型组合文库中鉴定高亲和力G蛋白偶联受体配体
7. Seamless Bead to Microarray Screening: Rapid Identification of the Highest Affinity Protein Ligands from Large Combinatorial Libraries [O] . Astle John M., Simpson Levi S., Huang Yong, 2010

机译：无缝珠子到微阵列筛选：大型组合图书馆中最高亲和力蛋白配体的快速鉴定

Identification of affinity ligands for protein purification from synthetic chemical combinatorial libraries

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