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首页> 外文期刊>Biotechnology Progress >Influence of carboxymethyl dextran and ferric citrate on the adhesion of CHO cells on microcarriers
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Influence of carboxymethyl dextran and ferric citrate on the adhesion of CHO cells on microcarriers

机译：羧甲基右旋糖酐和柠檬酸铁对CHO细胞在微载体上粘附的影响

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摘要

Due to the inherent risks of animal-derived raw materials, the biopharmaceutical industry has an increasing demand for serum-free and protein-free media for industrial cell culture bioprocesses. The absence of serum often changes the characteristics of mammalian cells, especially growth, productivity, and adherence properties. This study is mainly focused on the influence of media additives on cell adherence characteristics. An array of different carboxymethyl dextrans and different ferric citrate concentrations was tested with a number of CHO clones, using standard cell culture Roux-flasks and Cytoline 1 macroporous microcarriers. A prototype mixing system with controlled shear force input was developed as a screening system for adherence characteristics. The results of this evaluation revealed a negatively correlated dose-dependent influence on adhesion for ferric citrate. It was also found that certain carboxymethyl dextrans are capable of increasing the adherence on Roux-flasks and microcarriers.
机译：由于动物来源原材料的固有风险，生物制药行业对用于工业细胞培养生物过程的无血清和无蛋白质培养基的需求不断增长。血清的缺乏通常会改变哺乳动物细胞的特性，尤其是生长，生产力和粘附特性。这项研究主要集中于培养基添加剂对细胞粘附特性的影响。使用标准的细胞培养Roux瓶和Cytoline 1大孔微载体，用许多CHO克隆测试了一系列不同的羧甲基葡聚糖和不同浓度的柠檬酸铁。开发了具有受控剪切力输入的原型混合系统，作为粘附特性的筛选系统。该评估的结果表明，柠檬酸铁对黏附的剂量依赖性影响呈负相关。还发现某些羧甲基葡聚糖能够增加对Roux烧瓶和微载体的粘附。

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