Blends of natural polysaccharide sodium alginate (5%) with gelatin (3%) with gelation (5%) with gelation (3%) CROSS-linded with glutareldehyde provide heads with excellent compressive strength (8 x 10~4 Pa) and regular structure on treatment with calcium chloride.Lipases from prrcine pancreas Pseudomonas cepacia and Candida rugost were immobilized in such a blend with excellent effidciency The immobilized wnxymes were immobilized in such a blend with excellent efficiency.The immobilized enzymaes were stable and were reused sedveral times Without siginicant loss enzyme activity both in aqueous and reverse micellar media.The beads were functionluized with succinic anhyried to obtain beads with extra carboxylix acid groups.These functionalized beads were than successfully used for 7.4 fold prification of crude porcine pancrdeastic lipase in a simple operation of protein binding at pH 5 and release at pH 8.5.
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机译：天然海藻酸钠（5％）与明胶（3％）的混合物，凝胶化（5％）凝胶化（3％）交联的戊二醛，具有优异的抗压强度（8 x 10〜4 Pa）和规则的结构将猪胰腺的假单胞菌假单胞菌和皱纹假丝酵母的脂质固定在这种混合物中，效率极高。固定的酶在这种混合物中的固定效率极高。在水性和反胶态介质中都具有酶活性。将珠子用琥珀酸脱水官能化以获得带有额外羧基羧酸基团的珠子。这些功能化的珠子已成功用于7.4倍粗猪胰脂肪酶的简单纯化，其蛋白结合于pH 5并在pH 8.5释放。
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