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首页> 外文期刊>Biotechnology Progress >Effect of pH on Infectivity and Morphology of Ecotropic Moloney Murine Leukemia Virus
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Effect of pH on Infectivity and Morphology of Ecotropic Moloney Murine Leukemia Virus

机译：pH值对嗜性莫洛尼鼠白血病病毒感染性和形态的影响

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A number of factors affect the infectivity of retroviruses.The effect of pH on infectivity and morphology of ecotropic moloney muring leukemia virus (MoMuLV) was deter-mined in this work The ecotrpic MoMuLVs were found to remain infectious at a narrow pH RANGE from 5.5 to 8.0 Our experiments indicated that the virused were inactivated swiftly at lower or higher pH Within 5 minof esposure to pH 4 about 95% of the virused lost infctiousness.The viruses were completely inactivated after exposure to pH < 3 or pH >11 for 5 min he inactivation fo MoMuLV was irreversible Electron microscopy revealed that ectoropic MoMuLV remained found-shaped at pH between 7.0 and 5 They became irregular with a convex head at pH <4 At pH 2 virtually all virion particles were penetated by sytains cfausisng the accumulation of heavy metals inside the particles.The penetration fi heavy metal inside the particles indicated the disassoxiation of the lipid bilayer the virused at low pH A FACS-based screening starategy for selecting high-titer retrovirus producing cell lines is also presented in this report.
机译：影响逆转录病毒感染性的因素有很多。本研究确定了pH值对嗜温性单株性白血病病毒（MoMuLV）的感染性和形态的影响。发现经济型MoMuLV在5.5至5.5的窄pH范围内仍具有传染性。 8.0我们的实验表明，病毒在较低或较高的pH值下迅速灭活，在pH值为4的5分钟内约95％的病毒丧失了感染力。暴露于pH <3或pH> 11达5分钟后，病毒被完全灭活。 MoMuLV的失活是不可逆的电子显微镜观察发现，在pH值为7.0至5时，扇形MoMuLV仍呈形状。在pH <4时，它们变得不规则，凸头呈球形。在pH 2时，几乎所有病毒体颗粒都被甲tain渗透，这说明内部重金属的积累颗粒内部重金属的渗透表明，在低pH条件下被病毒感染的脂质双层的解离。本报告还介绍了选择高滴度逆转录病毒生产细胞的方法。

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