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首页> 外文期刊>Biotechnology Progress >Electrostatic encapsulation and growth of plant cell cultures in alginate
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Electrostatic encapsulation and growth of plant cell cultures in alginate

机译：海藻酸盐中植物细胞培养物的静电包封和生长

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摘要

The growth of callus tissue from African violets [Saintpaulia], encapsulated in alginate using electrostatics, was investigated as well as the mechanism of alginate droplet formation. Alginate microbeads as small as 500 (±50)μm in diameter could beproduced by electrostatic extrusion directly from a plastic syringe (1900 micron extrusion orifice), in the absence of a needle. Video analysis of the mechanism of electrostatic alginate droplet formation from the syringe showed the development of a Taylor cone-like droplet which extended to form a thin strand that then broke up into droplets. Autoclaving of the alginate/medium solution significantly reduced its viscosity, giving smaller beads. Calculated microbead diameters agreed well with experimental values. Callus tissue from leaf explants was successfully immobilized and cultured using electrostatic extrusion. Tissue immobilized using 4% alginate in medium and cultured on agar grew best, producing a complete plantlet within 4 months. The long-term aim is to develop an effective method for large production of artificial seeds.
机译：研究了用静电将藻类包裹在藻酸盐中的非洲紫罗兰[非洲堇]愈伤组织的生长以及藻酸盐液滴形成的机理。直径不超过500（±50）μm的藻酸盐微珠可通过无针注射器直接从塑料注射器（1900微米挤出孔口）直接静电挤出而制得。视频分析了注射器中形成的藻酸盐液滴的机理，显示出泰勒锥状液滴的形成，该液滴延伸形成细链，然后分解成液滴。藻酸盐/介质溶液的高压灭菌可显着降低其粘度，从而产生较小的珠粒。计算的微珠直径与实验值吻合得很好。来自叶片外植体的愈伤组织被成功地固定并使用静电挤压培养。在培养基中使用4％海藻酸钠固定并在琼脂上培养的组织生长最好，在4个月内产生完整的幼苗。长期目标是开发一种有效生产大量人工种子的方法。

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