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首页> 外文期刊>JIRCAS Working Report >A simple method for evaluation of salt tolerance and its application for screening of wild soybean (Glycine soja Sieb. & Zucc.) germplasm
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A simple method for evaluation of salt tolerance and its application for screening of wild soybean (Glycine soja Sieb. & Zucc.) germplasm

机译：一种简单评价耐盐性及筛选野生大豆（甘氨酸Soja Sieb。＆Zucc.）种质的方法

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摘要

A simple and effective method for evaluating hundreds of soybean genotypes is very useful for salt tolerance genetic studies and breeding. Afield evaluation can handle a large number of materials. However, the salt distribution has gradient either vertical or horizontal in a salinity field. This may result in an incorrect evaluation. Hydroponics can provide a reliable evaluation, however it is labor and cost consuming. In this report, we described a salt-water flooding method for evaluating salt tolerance for a large number of plant materials. When this method was applied to screening of 174 wild soybean (Glycine soja) accessions from Japan, several genotypes, such as JP30932 and JP30942, were identified to be salt tolerant. Evaluation of these soybean accessions using hydroponic method with a half strength of Hoagland and Arnon nutrient solution containing 100 mM NaCl gave a similar evaluation result as the salt-water flooding method did, indicating that the salt-water flooding method was simple and effective for screening large number of soybean genotypes, and it also had potential use in other crops. The salt tolerant wild soybean accessions, JP30932 and JP30942, might be used as genetic resources for improvement of salt tolerance in cultivated soybean.
机译：评估数百种大豆基因型的简单有效方法对于耐盐性遗传学研究和育种非常有用。远处评估可以处理大量材料。然而，盐分分布在盐度场中具有垂直或水平的梯度。这可能导致评估不正确。水培学能提供可靠的评估，但它是劳动力和成本耗费。在本报告中，我们描述了一种用于评估大量植物材料的耐盐性的盐水洪水方法。当该方法应用于从日本筛选174种野生大豆（甘氨酸SOJA）涂抹时，鉴定了几种基因型，例如JP30932和JP30942，以耐盐。利用含有100mM NaCl的HOAGLAND和ARNON营养溶液的半强度的水培法评价这些大豆饲料得到了类似的评价结果​​，随着盐水泛滥的方法确实如此，表明盐水洪水方法简单且有效地筛选大量大豆基因型，它也潜在地在其他作物中使用。耐盐野生大豆拆查，JP30932和JP30942可用作改善培养大豆耐盐性的遗传资源。

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  • 来源
    《JIRCAS Working Report》 |2011年第71期|共6页
  • 作者

    Donghe Xu; Hua Cong;

  • 作者单位

    Japan International Research Center for Agricultural Sciences (JIRCAS) Tsukuba Ibaraki 305-8686 Japan;

    Institute of Crop Germplasm Resource Xinjiang Academy of Agricultural Sciences No. 403 Nanchang Road Urumqi Xinjiang 830091 China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农业科学;
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