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首页> 外文期刊>Biotechnology Progress >Biokinetic Evaluation and Modeling of Continuous Thiocyanate Biodegradation by Klebsiella sp.
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Biokinetic Evaluation and Modeling of Continuous Thiocyanate Biodegradation by Klebsiella sp.

机译：克雷伯菌连续降解硫氰酸盐的生物动力学评估和建模。

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Biokinetics for autotrophic degradation of thiocyanate using batch culture of Klebsiella sp.were evaluated both analytically and numerically.A sequential approach with an analytical method followed by a numerical approximation was used to evaluate and to ensure the accuracy of the parameter estimation.The nonlinear least-squares method with a 95% confidence interval was employed.The growth conditions were maintained at pH 7 and 38 °C for all experiments.With an automated incubation and turbidity reader,a total of 16 different initial thiocyanate concentrations,ranging from 10 to 300 mg L~(-1),were used to develop a kinetic expression of specific growth rate as a function of substrate concentration.The biodegradation of thiocyanate with Klebsiella sp.followed a substrate inhibition pattern.Three identical automated bioreactors with working volumes of 1.5 L,equipped with sterilizable sampling ports,were also used for the numerical approximation of the biokinetic parameters in batch mode.A fourth order Runge-Kutta method was used to approximate the substrate inhibition kinetics of the Klebsiella sp.utilizing thiocyanate.Although the kinetic coefficients estimated by analytical and numerical methods were not statistically different at a 0.05 a level,model responses of numerical approximation generated a better prediction of changes in thiocyanate and cell mass concentrations.The hypothetical maximum growth rate,mu_m,half saturation coefficient,K_s,microbial yield coefficient,Y,cell mass decay rate coefficient,k_d,and substrate inhibition coefficient,K_(si),were evaluated as being 0.62+-0.05 d~(-1),85+-8 mg SCN~-L~(-1),0.076+-0.011 mg cell mass (mg SCN)~(-1),0.03 ± 0.002 d~(-1),and 131+-22 mg SCN~-L~(-1),respectively.The calculated maximal substrate concentration,S_m,and apparent maximum specific growth rate,mu'_m,were 105.5+-8.7 mg SCN~-L~(-1)and 0.24+-0.01 d~(-1),respectively.Using these estimated parameters,the theoretical performance of the continuous operation was also illustrated,which depicts the residual thiocyanate and Klebsiella sp.concentrations in the non-steady and steady states at different hydraulic retention times (HRTs).Assuming the influent concentration of 250 mg SCN~-L~(-1),the expected treatment efficiency ranged from 94.9% to 69.4% between 20 and 5 days HRT,respectively.Klebsiella sp.was expected to be washed out at 4.8 days HRT,thus resulting in no treatment of thiocyanate.
机译：对克雷伯菌属分批培养自养生物降解硫氰酸盐的生物动力学进行了分析和数值评估。采用顺序分析法和数值逼近法进行评估，以确保参数估计的准确性。使用置信区间为95％的平方方法。所有实验的生长条件均保持在pH 7和38°C下。使用自动孵育和浊度读取器，共有16种不同的初始硫氰酸盐浓度，范围从10到300 mg L〜（-1）被用来发展特定生长速率随底物浓度变化的动力学表达.Klebsiella sp。对硫氰酸盐的生物降解遵循底物抑制模式。三个相同的自动生物反应器，工作体积为1.5 L，配备了可消毒的采样口，还用于批处理模式下生物动力学参数的数值近似。使用四阶Runge-Kutta方法来近似利用硫氰酸盐对克雷伯菌的底物抑制动力学。尽管通过分析和数值方法估算的动力学系数在0.05 a水平上无统计学差异，但数值近似的模型响应产生了更好地预测了硫氰酸盐和细胞质量浓度的变化。假设的最大生长速率，mu_m，半饱和系数，K_s，微生物产量系数，Y，细胞质量衰减率系数，k_d和底物抑制系数K_（si）为评估为0.62 + -0.05 d〜（-1），85 + -8 mg SCN〜-L〜（-1），0.076 + -0.011 mg细胞质量（mg SCN）〜（-1），0.03±0.002 d 〜（-1）和131 + -22 mg SCN〜-L〜（-1）。计算出的最大底物浓度S_m和表观最大比生长率mu'_m为105.5 + -8.7 mg SCN 〜-L〜（-1）和0.24 + -0.01 d〜（-1）。使用这些估计参数，连续操作的理论性能为如下图所示，其描绘了在不同水力停留时间（HRT）下非稳态和稳态下的残留硫氰酸盐和克雷伯菌的浓度。假设进水浓度为250 mg SCN〜-L〜（-1），则预期的处理方法HRT在20至5天之间的效率分别为94.9％至69.4％。预计Klebsiella sp。将在HRT 4.8天被洗掉，因此将不进行硫氰酸盐的处理。

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