• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Effect of Subculture and Elicitation on Instability of Taxol Production in Taxus sp.Suspension Cultures
【24h】

Effect of Subculture and Elicitation on Instability of Taxol Production in Taxus sp.Suspension Cultures

机译：继代培养和诱导对紫杉悬浮培养物中紫杉醇生产不稳定的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The production of secondary metabolites through plant cell suspension cultures is challenging because the level and pattern of production is often unstable and unpredictable.To investigate the factors affecting instability of secondary metabolite production,high Taxol(paclitaxel)-producing Taxus cultures induced by methyl jasmonate elicitation and their low Taxol-producing counterparts were compared with respect to growth and Taxol production kinetics.With Taxus subcultures we observe alternating states of high and low productivity.Parental cultures and their subcultures from five different cell lines were used to test whether a high-producing culture grows more slowly or dies more rapidly than a low-producing one.These cell lines were of three types:(1)Taxol-producing with and without methyl jasmonate,(2)Taxol-producing only upon elicitation,and(3)nonproducing.High-producing cultures show growth inhibition upon subculture,whereas nonproducing elicited cultures show little growth inhibition.Thus,growth inhibition is primarily due to Taxol or taxane accumulation and not a direct result of methyl jasmonate treatment.Through media exchange between high- and low-producing cultures,it appears that culture components generated by cells alter culture properties.To assess variability as a function of culture lineage,two groups of replicate cultures were generated either with a mixing of the parental flasks or segregation of parental flasks at each subculture.Although parental culture mixing did not reduce flask-to-flask variation,the production level of Taxol in subcultures resulting from mixing inocula was sustained at a higher level relative to segregated subcultures.The results are consistent with the possibility of cell signaling within the population that can induce Taxol production.
机译：通过植物细胞悬浮培养产生次生代谢产物具有挑战性，因为其产生的水平和模式通常是不稳定且不可预测的。要研究影响次生代谢产物不稳定的因素，茉莉酸甲酯的诱导可导致高产紫杉醇（紫杉醇）产生的紫杉类培养物在生长和紫杉醇生产动力学方面比较了它们的低产紫杉醇。通过红豆杉亚培养物，我们观察到了高生产力和低生产力的交替状态。使用来自五个不同细胞系的亲本培养物及其亚培养物来测试高产这些细胞系分为三种类型：（1）有茉莉酸甲酯和无茉莉酸甲酯的情况下产生的紫杉醇;（2）仅在诱导时产生的紫杉醇;和（3）不产生的高产文化对继代培养表现出生长抑制作用，而非生产引起的文化则没有抑制生长因此，生长抑制主要归因于紫杉酚或紫杉烷的积累，而不是茉莉酸甲酯处理的直接结果。通过高产和低产培养物之间的培养基交换，看来细胞产生的培养物成分改变了培养物的性质。变异性随培养谱系的变化而变化，在每次传代培养时，通过亲本烧瓶的混合或亲本烧瓶的分离产生两组重复培养。尽管亲本培养混合并没有减少烧瓶到烧瓶的变异，相对于分离的亚培养物，混合接种产生的亚培养物中的紫杉醇维持在较高水平。结果与人群中细胞信号传导可能诱导紫杉醇产生的可能性相一致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号