High Temperature Increases the Refolding Yield of Reduced Lysozyme;Implication for the Productive Process for Folding

Ryusuke Sakamoto; Shingo Nishikori; Kentaro Shiraki

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High Temperature Increases the Refolding Yield of Reduced Lysozyme;Implication for the Productive Process for Folding

机译：高温提高了还原的溶菌酶的重折叠产量;对折叠生产过程的意义

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Misfolding poses a serious problem in the biotechnological field in obtaining the active protein from inclusion bodies.Here we show that high temperature increases the refolding yield of reduced lyosyzme by a simple dilution method.The refolding yields at 98 deg C were three times higher than those at 20 deg C in the solutions tested,which is related to the fact that the thermally unfolded state of lysozyme is a more productive form for folding than the denaturant-induced fully unfolded state.The thermal-assisted refolding could be used for various reduced and denatured proteins as a result of its simplicity and low cost.

机译：在生物技术领域中，错误折叠是从包涵体中获得活性蛋白的一个严重问题。在这里，我们证明了高温通过简单的稀释方法增加了还原的溶菌酶的重折叠产量.98摄氏度下的重折叠产量是其的三倍。在20°C的温度下，溶菌酶的热解折叠状态比变性剂诱导的完全解折叠状态更具生产性。热辅助重折叠可用于各种还原和还原反应。变性蛋白的简单性和低成本的结果。

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《Biotechnology Progress》 |2004年第4期|共6页
作者
Ryusuke Sakamoto; Shingo Nishikori; Kentaro Shiraki;
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中图分类生物工程学（生物技术）;
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1. High Temperature Increases the Refolding Yield of Reduced Lysozyme;Implication for the Productive Process for Folding [J] . Ryusuke Sakamoto, Shingo Nishikori, Kentaro Shiraki Biotechnology Progress . 2004,第4期

机译：高温提高了还原的溶菌酶的重折叠产量;对折叠生产过程的意义
2. Refolding studies using pressure: The folding landscape of lysozyme in the pressure-temperature plane [J] . Smeller L, Meersman F, Heremans K Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics . 2006,第3期

机译：使用压力的重折叠研究：溶菌酶在压力-温度平面中的折叠情况
3. Analysis of the early stage of the folding process of reduced lysozyme using all lysozyme variants containing a pair of cysteines. [J] . Shioi S, Imoto T, Ueda T Biochemistry . 2004,第18期

机译：使用所有含有一对半胱氨酸的溶菌酶变异体，对减少的溶菌酶折叠过程的早期阶段进行分析。
4. Refolding of reduced lysozyme using weak cation exchange chromatography [C] . International Beijing conference and exhibition on instrumental analysis;BCEIA 2003 . 2003

机译：使用弱阳离子交换色谱法还原还原的溶菌酶
5. The adaptation of Chinese hamster ovary cells to hypothermic temperatures increases yields of monomeric recombinant interferon-beta. [D] . Sunley, Kevin Michael. 2009

机译：中国仓鼠卵巢细胞适应低温的温度增加了单体重组干扰素-β的产量。
6. Temperature increase reduces global yields of major crops in four independent estimates [O] . Chuang Zhao, Bing Liu, Shilong Piao, 2017

机译：温度升高使四个独立估计值降低了主要作物的全球单产
7. 2P095 Argininamide : New class of additive that improves the refolding yield of lysozyme(31. Protein folding and misfolding (II),Poster Session,Abstract,Meeting Program of EABS BSJ 2006) [O] . Hiroyuki Hamada, Kentaro Shiraki 2006

机译：2P095氨基氨基酰胺：新的添加剂，提高溶菌酶重折叠产量（31.蛋白质折叠和错配（II），海报会议，摘要，EABS＆BSJ 2006的会议计划）

High Temperature Increases the Refolding Yield of Reduced Lysozyme;Implication for the Productive Process for Folding

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