Sequence-Specific Oligonucleotide Purification Using Peptide Nucleic Acid Amphiphiles in Hydrophobic Interaction Chromatography

James P.Vernille; James W.Schneider

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Sequence-Specific Oligonucleotide Purification Using Peptide Nucleic Acid Amphiphiles in Hydrophobic Interaction Chromatography

机译：疏水相互作用色谱中使用肽核酸两亲物纯化特定序列的寡核苷酸

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We present a methodology to perform sequence-specific separations of oligonucleotides using pep tide nucleic acids covalently linked to alkane chains,or "PNA amphiphiles(PNAAs)".The PNAA/DNA duplexes are discriminated from unbound DNA using hydrophobic interaction chromatography on a phenyl-substituted Sepharose column.Nearly quantitative recovery is achieved at concentrations of 50 muM after incubation of oligomers with a stoichiometric amount of PNAA for 1 min or so.The method exhibits high sequence specificity,selectivity,and resolution when applied to mixtures of various oligomers up to 60 base pairs in length.

机译：我们提出了一种使用共价连接到烷烃链或“ PNA两亲物（PNAAs）”的肽段核酸进行寡核苷酸序列特异性分离的方法。使用苯基上的疏水相互作用色谱法将PNAA / DNA双链体与未结合的DNA区别开来。将低聚物与化学计量的PNAA孵育1分钟左右后，在50μM的浓度下几乎可以实现定量回收。该方法在将各种低聚物混合使用时，具有很高的序列特异性，选择性和分离度长度为60个碱基对。

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《Biotechnology Progress》 |2004年第6期|共7页
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James P.Vernille; James W.Schneider;
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中图分类生物工程学（生物技术）;
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1. Sequence-Specific Oligonucleotide Purification Using Peptide Nucleic Acid Amphiphiles in Hydrophobic Interaction Chromatography [J] . James P.Vernille, James W.Schneider Biotechnology Progress . 2004,第6期

机译：疏水相互作用色谱中使用肽核酸两亲物纯化特定序列的寡核苷酸
2. A theoretical analysis of specificity of nucleic acid interactions with oligonucleotides and peptide nucleic acids (PNAs) [J] . Lomakin A., Frank-Kamenetskii MD. Journal of Molecular Biology . 1998,第1期

机译：对与寡核苷酸和肽核酸（PNA）相互作用的核酸的特异性的理论分析
3. Peptide nucleic acid probe for protein affinity purification based on biotin-streptavidin interaction and peptide nucleic acid strand hybridization [J] . Tse Jenny, Wang Yuanyuan, Zengeya Thomas, Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2015,第Null期

机译：基于生物素-链霉亲和素相互作用和肽核酸链杂交的蛋白亲和纯化肽核酸探针
4. Sequence-Specific Cleavage of DNA by Peptide Nucleic Acids Conjugated with Metal Complexes [C] . Toru Sugiyama, Keiko Ninomiya, Yasutada Imamura Japanese peptide symposium . 2010

机译：用金属络合物缀合的肽核酸的DNA序列特异性切割
5. Sequence-specific DNA purification using peptide nucleic acid amphiphiles. [D] . Vernille, James Peter. 2004

机译：使用肽核酸两亲物进行序列特异性DNA纯化。
6. Sequence-Specific Purification of DNA Oligomers in Hydrophobic Interaction Chromatography Using Peptide Nucleic Acid Amphiphiles: Extended Dynamic Range [O] . Jeffrey M. Savard, James W. Schneider -1

机译：疏水相互作用色谱中使用肽核酸两亲物的DNA低聚物的序列特定纯化：扩展的动态范围
7. N-(2-Hydroxyethyl)phenazinium derivatives of oligonucleotides as effectors of the sequence-specific modification of nucleic acids with reactive oligonucleotide derivatives [O] . Kutyavin I.V., Podyminogin M.A., Bazhina Yu.N., 1988

机译：寡核苷酸的N-（2-羟乙基）吩嗪鎓衍生物作为反应性寡核苷酸衍生物对核酸进行序列特异性修饰的效应子

Sequence-Specific Oligonucleotide Purification Using Peptide Nucleic Acid Amphiphiles in Hydrophobic Interaction Chromatography

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