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首页> 外文期刊>Biotechnology Progress >Enhanced Lycopene Productivity by Manipulation of Carbon Flow to Isopentenyl Diphosphate in Escherichia coli
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Enhanced Lycopene Productivity by Manipulation of Carbon Flow to Isopentenyl Diphosphate in Escherichia coli

机译：通过操纵碳流向大肠杆菌中的异戊烯基二磷酸酯流动来提高番茄红素生产率

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摘要

Lycopene is a useful phytochemical that holds great commercial value.In our study the lycopene production pathway in E.coli originating from the precursor isopentenyl diphosphate (IPP) of the non-mevalonate pathway was reconstructed.This engineered strain of E.coli accumulated lycopene intracellularly under aerobic conditions.As a next step,the production of lycopene was enhanced through metabolic engineering methodologies.Various competing pathways at the pyruvate and acetyl-CoA nodes were inactivated to divert more carbon flux to IPP and subsequently to lycopene.It was found that the ackA-pta,nuo mutant produced a higher amount of lycopene compared to the parent strain.To further enhance lycopene production,a novel mevalonate pathway,in addition to the already existing non-mevalonate pathway,was engineered.This pathway utilizes acetyl-CoA as precursor,condensing it to form acetoacetyl-CoA and subsequently leading to formation of IPP.Upon the introduction of this new pathway,lycopene production increased by over 2-fold compared to the ackA-pta,nuo mutant strain.
机译：番茄红素是一种有用的植物化学物质，具有重要的商业价值。下一步，通过代谢工程学方法提高了番茄红素的生产。丙酮酸和乙酰辅酶A节点上的各种竞争途径被灭活，从而将更多的碳通量转移到IPP和随后的番茄红素上。 ackA-pta，nuo突变体比亲本菌株产生了更多的番茄红素。为了进一步提高番茄红素的产生，除了已经存在的非甲羟戊酸途径外，还设计了一种新颖的甲羟戊酸途径。该途径利用乙酰辅酶A作为前体，将其冷凝形成乙酰乙酰辅酶A，随后导致IPP的形成。与ackA-pta，nuo突变菌株相比，产量增加了2倍以上。

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