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首页> 外文期刊>Biotechnology Progress >Influence of auxins and sucrose in monoterpenoid oxindole alkaloid production by Uncaria tomentosa cell suspension cultures.
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Influence of auxins and sucrose in monoterpenoid oxindole alkaloid production by Uncaria tomentosa cell suspension cultures.

机译：生长素和蔗糖对毛白钩虫细胞悬浮培养物生产单萜类羟吲哚生物碱的影响。

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Growth and alkaloid production in Uncaria tomentosa cell suspension cultures were studied in Murashige and Skoog medium supplemented with 10 micro M 2,4-dichlorophenoxyacetic acid, 10 micro M kinetin, and 58 mM sucrose for maintenance and with 10 micro M indole-3-acetic acid, 10 micro M kinetin, and 58 mM sucrose for production. A U. tomentosa pale Uth-3 cell line, cultured in the production medium, showed a reduced lag phase and a specific growth rate ( mu ) of 0.27 day-1, while cells growing in the maintenance medium showed mu =0.20 day-1. U. tomentosa cells growing in the production medium produced monoterpenoid oxindole alkaloids (MOA) in amounts of 10.2+or-1.6 micro g g-1 dry weight (DW). The chemical profile of MOA produced by in vitro cell cultures was similar to that found in the plant. After 10 subcultures, maximum MOA production decreased to 2.0+or-0.7 micro g g-1 DW, while tryptamine alkaloids (TA) were produced with a maximum of 6.2+or-0.4 micro g g-1 DW. The increase of initial sucrose concentration up to 145 mM in the production medium enhanced the cell biomass by 3.2-fold (from 10.2+or-0.1 to 32.8+or-1.1 g DW L-1), reduced mu from 0.27 to 0.23 day-1, and provoked a substantial accumulation of TA (23.1+or-4.7 micro g g-1 DW). A high sucrose concentration stimulated MOA production in the maintenance medium (2.7+or-0.5 micro g g-1 DW), even in the presence of 2,4-dichlorophenoxyacetic acid.
机译：在补充10 micro M 2,4-二氯苯氧基乙酸，10 micro M激动素和58 mM蔗糖以维持维持性和10 micro M吲哚-3-乙酸的Murashige和Skoog培养基中研究了毛圆钩虫细胞悬浮培养物中的生长和生物碱的产生。酸，10 micro M激动素和58 mM蔗糖进行生产。在生产培养基中培养的毛绒线虫淡Uth-3细胞系显示出减少的迟滞期和0.27 day-1的比生长速率（mu），而在维持培养基中生长的细胞显示mu = 0.20 day-1 。在生产培养基中生长的毛绒球菌细胞产生的单萜类羟吲哚生物碱（MOA）的干重为10.2 +或-1.6 micro g g-1。体外细胞培养产生的MOA的化学特征与植物相似。经过10次传代培养后，最大MOA产量降至2.0+或-0.7 micro g g-1 DW，而色胺碱生物碱（TA）的最大产量为6.2+或-0.4 micro g g-1 DW。生产培养基中初始蔗糖浓度增加至145 mM可使细胞生物量增加3.2倍（从10.2+或-0.1增至32.8+或-1.1 g DW L-1），将mu从0.27减少至0.23天- 1，并引起大量的TA（23.1 +或-4.7 micro g g-1 DW）积累。即使在存在2,4-二氯苯氧基乙酸的情况下，高蔗糖浓度也会在维持培养基（2.7+或-0.5 micro g g-1 DW）中刺激MOA的产生。

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