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首页> 外文期刊>Biotechnology Progress >Enzymatic Hydrolysis of Soluble Starch with an a-Amylase from Bacillus licheniformis
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Enzymatic Hydrolysis of Soluble Starch with an a-Amylase from Bacillus licheniformis

机译：地衣芽孢杆菌α-淀粉酶对可溶性淀粉的酶解作用

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The enzymatic hydrolysis of soluble starch with an alpha-amylase from Bacillus licheniformis (commercial enzyme Termamyl 300 L Type DX)have been experimentally studied at pH 7.5,within the temperature range of 37-75 deg C,at initial substrate concentrations of between 0.25 and 2.00 g/L,and enzyme concentrations of between 0.575 x 10~(-4)and 13.8 x 10~(-4)g/L.To follow the reaction a procedure based on the iodometric method for measuring alpha-amylase activity was used.The kinetics of the enzymatic hydrolysis was fitted to the Michaelis-Menten equation using the integral method,taking into account that the thermal deactivation of the enzyme follows a second-order kinetic.These parameters were fitted to the Arrhenius equation obtaining activation energies of 24.4 and 41.7 kJ/mol and preexponential factors of 734.9 g/L and 1.74 x 10~8 min~(-1)for K_M and k,respectively.
机译：已经对地衣芽孢杆菌（商品酶Termamyl 300 L DX型）中的α-淀粉酶进行了可溶性淀粉的酶水解实验，pH值为7.5，温度范围为37-75℃，初始底物浓度为0.25-0.5℃。 2.00 g / L，酶浓度在0.575 x 10〜（-4）和13.8 x 10〜（-4）g / L之间。为了跟踪反应，使用了基于碘量法的方法来测量α-淀粉酶活性考虑到酶的热失活遵循二级动力学，使用积分方法将酶解的动力学拟合为Michaelis-Menten方程，将这些参数拟合为Arrhenius方程，获得24.4的活化能K_M和k分别为41.7 kJ / mol和734.9 g / L和1.74 x 10〜8 min〜（-1）的指数前因子。

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