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首页> 外文期刊>Journal of Analytical Toxicology >Application of a Urine and Hair Validated LC-MS-MS Method to Determine the Effect of Hair Color on the Incorporation of 25B-NBOMe, 25C-NBOMe and 25I-NBOMe into Hair in the Rat
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Application of a Urine and Hair Validated LC-MS-MS Method to Determine the Effect of Hair Color on the Incorporation of 25B-NBOMe, 25C-NBOMe and 25I-NBOMe into Hair in the Rat

机译：尿液验证的LC-MS-MS方法的应用来确定毛发颜色对大鼠致氢毛发的掺入致氢的影响

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NBOMes are a group of new psychoactive substances derived from phenethylamines. Recreational abuse is thought to have begun in 2010 and they are commonly associated with the "club drug" scene. They are administered in liquid form or as blotters due to their high potency. An LC-MS-MS method was validated using Scientific Working Group for Forensic Toxicology parameters for the detection of 25B-, 25C- and 4-iodo-2,5-dimethoxy-N-[(2-methoxyphenyl) methyl]-benzeneethanamine (25I-NBOMe) using 4-bromo-2,5-dimethoxy-N-[(2-methoxyphenyl) methyl]-benzeneethanamine (25B-NBOMe)-D-3 as internal standard for urine and hair. Calibration graphs with R-2 values > 0.99 were observed for urine and hair for concentrations ranging from 0.1 to 100 ng/mL and 0.025 to 2.5 ng/mg, respectively. Urine LODs ranged from 5 to 25 pg/mL and had an LOQ of 50 pg/mL. Hair LOD and LOQs ranged from 3 to 5 pg/mg and 6.25 to 12.5 pg/mg, respectively. Intra- and inter-day precision was <20% and accuracy was within +/- 20% for both matrices. The method was shown to be selective for both exogenous and endogenous compounds. No matrix effects were observed for either matrix. LLE recovery ranged from 90 to 103% for urine samples and solid phase extraction recovery ranged from 80 to 107% for hair samples. Long-Evans rats (n = 55) were administered 25B-, 25C-or 25I-NBOMe at doses ranging from 30 to 300 mu g/kg over a period of 10 days. Rats were shaved prior to their first dose and re-shaved after the 10-day period. Hair was separated by color (black: n = 55 and white: n = 55) and analyzed using the validated LC-MS-MS method to assess the impact hair color has on the incorporation of these drugs. All drugs were successfully detected in black hair. 25B-NBOMe from rats receiving the highest dose and 25C-NBOMe from rats receiving the medium and high doses were quantified in white hair. 25I-NBOMe was detected but fell below the limit of quantification. A dose-dependent concentration increase was observed in the black hair. All pooled urine samples tested positive for their expected NBOMes.
机译：Nbomes是衍生自苯乙胺的一组新的精神活性物质。娱乐虐待被认为已经开始于2010年开始，它们通常与“俱乐部药物”场景有关。它们以较高的效力以液体形式或作为暗形施用。使用科学工作组验证LC-MS-MS方法，用于检测25b-，25℃和4-碘-2,5-二甲氧基-N - [（2-甲氧基苯基）甲基] - 苯苯胺（ 25i-nbome）使用4-溴-2,5-二甲氧基-N - [（2-甲氧基苯基）甲基] - 苯苯胺（25b-nbome）-d-3作为尿液和毛发的内标。含有R-2值> 0.99的校准图对于尿液和浓度分别为0.1至100ng / ml和0.025至2.5ng / mg的浓度。尿液的范围为5至25pg / ml，并具有5​​0pg / ml的LOQ。毛发床和LOQ分别从3至5 pg / mg和6.25至12.5 pg / mg。在日内和日间精度<20％，并且两种矩阵的精度在+/- 20％内。该方法显示为外源和内源化合物选择性。对于任一矩阵没有观察到矩阵效应。尿液样品的90至1​​03％的lex恢复范围从80〜107％的头发样品的范围。在10天内以30-300μg/ kg的剂量施用25b-，25℃或25i-nbome的长evans大鼠（n = 55）。在他们的第一次剂量之前剃掉了大鼠并在10天后重新剃。头发被颜色分开（黑色：n = 55和白色：n = 55），并使用经过验证的LC-MS-MS方法进行分析以评估冲击毛发对这些药物的掺入。所有药物在黑发中成功检测到。从接受从接受培养基和高剂量的大鼠接收最高剂量和25℃-NBome的大鼠的25b-nbome被白发定量。检测到25i-nbome但低于量化限制。在黑发中观察到剂量依赖性浓度增加。所有合并的尿液样本都针对预期的Nbomes测试了阳性。

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