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首页> 外文期刊>Journal of Animal Physiology and Animal Nutrition >Adaptation of aerobic training essentially involved autophagy, mitochondrial marker and muscle fibre genetic modulation in rat cardiac muscles
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Adaptation of aerobic training essentially involved autophagy, mitochondrial marker and muscle fibre genetic modulation in rat cardiac muscles

机译：适应有氧训练基本上涉及大鼠心肌肌肉的自噬，线粒体标记和肌纤维遗传调制

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Information about the role of moderate acute treadmill training in modulating autophagy and mitochondrial markers that might be correlated with alteration of muscle fibre gene expression in rat cardiac muscles is very limited. In this present study, the researchers divided twenty male Wistar rats into four groups: sedentary control, 3, 6 and 15 days and subjected them to treadmill training with moderate intensity (20 m/min), 30 min each day. RNA was extracted from cardiac muscles and stored in temperature of -80 degrees C. Specific primers were utilized for semi-quantitative PCR. Treadmill training decreased autophagy-related gene expression (LC3, p62) and upper stream signalling of autophagy (PIK3CA, Akt and mTOR) in 3 and 6 d, but stimulated gene expression of mitochondrial markers (PGC1 alpha, Cox1, Cox2 and Cox4) in 15 days. alpha MHC gene expression increased while beta MHC gene expression decreased in 15 days. In line with this, autophagy-related genes increased in 3 and 6 days and returned to baseline in 15 days. The increment in mitochondrial gene expression might be correlated with shifting gene expression of alpha MHC and beta MHC in 15 days. Taken together, acute adaptation in cardiac muscles is stimulated by genetic modulation of autophagy, mitochondrial marker and muscle fibre that may explain physiological cardiac adaptation after training. This study can be used as a reference for optimizing performance in period of cardiac muscle adaptation stimulated by treadmill training.
机译：适用于中等急性跑步机训练在调节动物和线粒体标记中的作用，这些标记可能与大鼠心肌肌肉的肌纤维基因表达的改变相关的自噬和线粒体标记非常有限。在本研究中，研究人员将20只雄性Wistar大鼠分为四组：久坐不动控制，3,6和15天，并使它们以中等强度（20米/分钟），每天30分钟跑步训练。从心肌肌肉中提取RNA，并储存在-80℃的温度下。使用特异性引物进行半定量PCR。跑步机训练减少了3和6天的自噬相关基因表达（LC3，P62）和自噬（PIK3CA，AKT和MTOR）的上游信号，但刺激了线粒体标记物的基因表达（PGC1α，COX1，COX2和COX4） 15天。 αMHC基因表达在βMHC基因表达中增加，在15天内降低。根据这一点，与自噬相关基因在3和6天内增加，并在15天内返回基线。线粒体基因表达中的增量可能与15天内αMHC和βMHC的移位基因表达相关。连胜，通过可解释训练后的生理心脏适应的自噬，线粒体标记和肌纤维的遗传调节刺激心肌中的急性适应。该研究可作为优化由跑步机训练刺激的心肌适应期间性能的参考。

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