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首页> 外文期刊>Journal of Analytical Methods in Chemistry >HPLC Method for Quantification of Caffeine and Its Three Major Metabolites inHumanPlasma Using Fetal Bovine Serum Matrix to Evaluate Prenatal Drug Exposure
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HPLC Method for Quantification of Caffeine and Its Three Major Metabolites inHumanPlasma Using Fetal Bovine Serum Matrix to Evaluate Prenatal Drug Exposure

机译：胎儿牛血清基质评价产前药物暴露的咖啡因含咖啡因及其三大代谢物的咖啡因及其三大代谢物的方法

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Caffeine is recognized as the first-line therapeutic agent for apnea of prematurity. +e dosage regimen is 10 mg/kg loading dose and 2.5 mg/kg maintenance dose. However, the plasma concentration achieved, not always, is therapeutically useful. It makes necessary to increase the doses to reach plasma concentration up to 30 or 35 μg/mL or even higher to attain therapeutic effect. To study why neonates have these differences, and whether these effects are linked to prenatal caffeine exposure, we had to develop an analytical method for an accurate measurement of caffeine and metabolites concentration. +e analysis was carried out using fetal bovine serum (FBS) as biological matrix in a high-performance liquid chromatography with an ultraviolet detector method. +is method allows acceptable chromatographic resolution between analytes in 15 minutes. It was validated and proved to be linear in the 0.1-40 μg/mL range for caffeine, paraxanthine, theobromine, and theophylline in the same chromatographic analysis. Accuracy for quality control samples for intra- and interday assays was ranged from 96.5 to 105.2% and 97.1 to 106.2%. Precision had CV no more than 10% in all concentration levels for all analytes. No differences were observed between quantification in human and FBS. +is method was applied to quantify plasma drug concentration in mothers and their newborns in a Mexican northeast population. In our study, we confirmed self-reported caffeine maternal intake in 85.2% (n = 23); meanwhile, in their newborn's plasma, it was detected only in 78% (n = 21). Caffeine plasma concentrations in mother and newborn had a linear relationship, and no differences were observed between groups (mothers versus children). +ese results suggest that our analytical method and substitution of biological matrix was linear, precise, and accurate for caffeine quantification and could be used for measuring prenatal exposure and let us to study, in the future, concentration differences observed du
机译：咖啡因被认为是前一种呼吸暂停的一线治疗剂。 + E剂量方案为10 mg / kg负载剂量和2.5mg / kg维持剂量。然而，实现的等离子体浓度并不总是治疗有用。需要增加剂量，以达到高达30或35μg/ ml甚至更高的血浆浓度以获得治疗效果。为什么新生儿具有这些差异，以及这些效果是否与产前咖啡因暴露有关，我们必须开发一种准确测量咖啡因和代谢物浓度的分析方法。 + e分析使用胎儿牛血清（FBS）作为生物基质，其高性能液相色谱法，具有紫外线检测方法。 +是方法在15分钟内允许分析物之间的可接受色谱分辨率。经过验证，并证明是在同一色谱分析中的咖啡因，帕雷氏曲线，茶碱和茶碱中的0.1-40μg/ ml范围内线性。用于质量控制样品的准确性，用于间隔和间隔测定的样品的范围为96.5至105.2％和97.1至106.2％。所有分析物的所有浓度水平的精度都没有超过10％。人和FBS的定量之间没有观察到差异。 +是含有墨西哥东北人群母亲及其新生儿的量化血浆药物浓度的方法。在我们的研究中，我们确认了85.2％的自我报告的咖啡因产妇摄入量（n = 23）;同时，在他们的新生儿的血浆中，它仅在78％（n = 21）中被检测到。母细胞膜血浆浓度在母亲和新生儿具有线性关系，群体之间没有观察到差异（母亲与儿童）。 + ESE结果表明，我们的分析方法和生物基质的取代是线性，精确，准确的咖啡因定量，可用于测量产前暴露，并让我们在未来观察到浓度差异杜

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