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首页> 外文期刊>Journal of Apicultural Research >Antimicrobial activity of allicin against honey bee pathogens
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Antimicrobial activity of allicin against honey bee pathogens

机译:含蜂蜜蜜蜂病原体的抗菌活性

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Allicin is the chief antimicrobial compound produced in garlic. It has been studied for activities against human and food-born pathogens. In this study the antimicrobial activity of allicin (Allisure(TM) liquid) was tested against a number of bacterial and fungal pathogens (Paenibacillus larvae larvae, P. I. pulvifaciens, Ascosphaera apis and Ascosphaera aggregata) associated with social (Apis mellifera) and solitary bees. The minimum inhibitory concentrations (MIC) of allicin were determined using a broth microdilution method in the range of 1000 ppm to 25 ppm. Allicin liquid showed activity against gram-positive bacterial isolates (MIC 350 ppm) and fungal isolates (MIC 250 ppm). The antimicrobial activity of allicin was also tested in an agar diffusion test using 250 mug of allicin per disk. Bacterial isolates (P. I. pulvifaciens and P. I. larvae) were associated with zones of inhibition in the range of 24-26 mm and 45-50 mm, respectively. The fungal isolates were associated with zones of inhibition in the range of 31-35 mm (A. apis) and 35-37 mm (A. aggregata). The macrolide class antibiotic tylosin (Tylan(R)50, Elanco Inc., IN) was used as a control in both the MIC assay and in the agar diffusion test. The data from this study point to the potential of allicin to inhibit growth of bee pathogens and reduce occurrence of at least two major bee diseases.
机译:AlliCIN是在大蒜中产生的主要抗微生物化合物。已经研究了针对人类和食物出生的病原体的活动。在这项研究中,抗菌素(暗区(TM)液体)对许多细菌和真菌病原体(Paenibacillus幼虫,P.I.Pulvifaciens,Ascoshaera Apis和Ascosphaera Andegata)进行了测试,与社会(Apis mellifera)和孤独蜜蜂相关。使用1000ppm至25ppm的肉汤微量稀释方法测定含有血小霉素的最小抑制浓度(MIC)。 AlliCIN液体显示针对革兰氏阳性细菌分离株(MIC 350ppm)和真菌分离物(MIC 250ppm)的活性。在每盘的250杯含有250杯丙蛋白中还在琼脂扩散试验中测试了含有含量的抗微生物活性。细菌分离株(P.I. pulvifaciens和P.i.Larvae)分别与24-26mm和45-50mm的抑制区相关。真菌分离物与抑制区的区域相关联,在31-35mm(A.API)和35-37mm(A. Aggregata)的范围内。 Macroolide类抗生素替斯汀(Tylan(R)50,Elanco Inc.,IN)用作MIC测定和琼脂扩散试验中的对照。本研究表明,含有含有蜂蛋白病原体生长的潜力,并减少至少两种主要蜂疾病的发生。

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