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首页> 外文期刊>Journal of applied toxicology >Regulation of NRF2, AP-1 and NF-kB by cigarette smoke exposure in three-dimensional human bronchial epithelial cells
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Regulation of NRF2, AP-1 and NF-kB by cigarette smoke exposure in three-dimensional human bronchial epithelial cells

机译：三维人支气管上皮细胞中的香烟烟雾暴露的NRF2，AP-1和NF-KB调节

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摘要

Cigarette smoke (CS) is a complex mixture of chemicals and interacts with various physiological processes. We previously reported that nuclear factor erythroid 2-related factor 2 (NRF2) was the most sensitive transcription factor to aqueous CS extract (AqCSE) exposure in monolayer cultured human bronchial epithelial cell lines. Recently, in vitro three-dimensional (3D) culture models have been used to supplement pharmacological and toxicological assessments. Bronchial epithelium models in particular are useful for the evaluation of substances that directly contact the respiratory tract, such as CS. In the present study, we used 3D-cultured human bronchial epithelial cells (HBECs) to assess activation of transcription factors and relevant gene expression in response to AqCSE, primarily focusing on NRF2 and nuclear factor-kappa B (NF-kB) pathways. The 3D-cultured HBECs exposed to AqCSE showed expression of NRF2 and its nuclear translocation in addition to upregulation of genes related to oxidative stress.
机译：香烟烟雾（CS）是化学品的复杂混合物，并与各种生理过程相互作用。我们以前报道，核因子红外2相关因子2（NRF2）是单层培养的人支气管上皮细胞系中CS提取物（AQCSE）暴露的最敏感的转录因子。最近，在体外三维（3D）培养模型已被用于补充药理学和毒理学评估。特别是支气管上皮模型对于评估直接接触呼吸道的物质，例如Cs。在本研究中，我们使用3D培养的人支气管上皮细胞（HBEC）来评估转录因子和相关基因表达的激活，并响应AQCSE，主要关注NRF2和核因子-Kappa B（NF-KB）途径。除了与氧化应激相关的基因的上调之外，暴露于AQCSE暴露于AQCSE的3D培养的HBEC表达NRF2及其核易位。

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