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首页> 外文期刊>Journal of applied toxicology >The effects of different bisphenol derivatives on oxidative stress, DNA damage and DNA repair in RWPE‐1 cells: A comparative study
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The effects of different bisphenol derivatives on oxidative stress, DNA damage and DNA repair in RWPE‐1 cells: A comparative study

机译：不同双酚衍生物对RWPE-1细胞中氧化应激，DNA损伤和DNA修复的影响：对比研究

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Abstract Bisphenol A (BPA) is a well‐known endocrine disruptor and it is widely used mainly in the plastics industry. Due to recent reports on its possible impact on health (particularly on the male reproductive system), bisphenol F (BPF) and bisphenol S (BPS) are now being used as alternatives. In this study, RWPE‐1 cells were used as a model to compare cytotoxicity, oxidative stress‐causing potential and genotoxicity of these chemicals. In addition, the effects of the bisphenol derivatives were assessed on DNA repair proteins. RWPE‐1 cells were incubated with BPA, BPF, and BPS at concentrations of 0–600 μM for 24 h. The inhibitory concentration 20 (IC 20 , concentration that causes 20% of cell viability loss) values for BPA, BPF, and BPS were 45, 65, and 108 μM, respectively. These results indicated that cytotoxicity potentials were ranked as BPA BPF BPS. We also found alterations in superoxide dismutase, glutathione peroxidase and glutathione reductase activities, and glutathione and total antioxidant capacity in all bisphenol‐exposed groups. In the standard and modified Comet assay, BPS produced significantly higher levels of DNA damage vs the control. DNA repair proteins (OGG1, Ape‐1, and MyH) involved in the base excision repair pathway, as well as p53 protein levels were down‐regulated in all of the bisphenol‐exposed groups. We found that the BPA alternatives were also cytotoxic and genotoxic, and changed the expressions of DNA repair enzymes. Therefore, further studies are needed to assess whether they can be used safely as alternatives to BPA or not.
机译：摘要双酚A（BPA）是一种众所周知的内分泌破坏器，其广泛应用于塑料行业。由于最近关于其对健康的影响（特别是在雄性生殖系统上的影响），现在使用双酚F（BPF）和双酚S（BPS）作为替代方案。在该研究中，使用RWPE-1细胞作为模型，以比较细胞毒性，氧化应激导致这些化学品的潜在和遗传毒性。此外，对DNA修复蛋白评估双酚衍生物的影响。将RWPE-1细胞与BPA，BPF和BPS一起温育0-600μm的浓度为24小时。 BPA，BPF和BPS的抑制浓度20（IC 20，导致20％的细胞活力损失的浓度）分别为45,65和108μm。这些结果表明，细胞毒性潜力被排名为BPA＆ bpf＆ BPS。我们还发现超氧化物歧化酶，谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性的改变，以及所有双酚暴露基团的谷胱甘肽和总抗氧化能力。在标准和改性的彗星测定中，BPS产生显着较高水平的DNA损伤VS对照。在所有双酚暴露的基团中，DNA修复蛋白（OGG1，APE-1和MYH）以及P53蛋白水平下调。我们发现BPA替代品也是细胞毒性和遗传毒性，并改变了DNA修复酶的表达。因此，需要进一步的研究来评估它们是否可以安全地用作BPA的替代品。

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