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首页> 外文期刊>Journal of applied toxicology >Differential effect of methyl-, butyl- and propylparaben and 17 beta-estradiol on selected cell cycle and apoptosis gene and protein expression in MCF-7 breast cancer cells and MCF-10A non-malignant cells
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Differential effect of methyl-, butyl- and propylparaben and 17 beta-estradiol on selected cell cycle and apoptosis gene and protein expression in MCF-7 breast cancer cells and MCF-10A non-malignant cells

机译：甲基，丁基和丙基和丙基和17β-雌二醇对MCF-7乳腺癌细胞和MCF-10A非恶性细胞中选定细胞周期和凋亡基因和蛋白表达的差异作用

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Parabens are alkyl esters of p-hydroxybenzoic acid used widely as antimicrobial preservatives in consumer products, including pharmaceuticals, foods and cosmetics. We showed previously that methyl-, butyl- and propylparaben parabens, even at low doses, stimulate the proliferation of MCF-7 breast cancer cells and non-transformed MCF-10A breast epithelial cells. The present study was undertaken to determine whether this represents a direct effect on cell cycle and apoptotic gene expression. MCF-7 and MCF-10A cells were exposed to methyl, butyl-and propylparaben (20 nM) or 17 beta-estradiol (10 nM). Cell cycle and apoptotic gene expression were evaluated by real-time polymerase chain reaction and protein expression by Western blot. 17 beta-estradiol upregulated G(1)/S phase genes and downregulated cell cycle progression inhibitors in both MCF-7 and MCF-10A. Upregulation of Bcl-xL and downregulation of caspase 9 was observed in MCF-7, while upregulation of Bcl-xL, BCL2L2 and caspase 9 was noted in MCF-10A. Cyclins in MCF-7 cells were not affected by any of the parabens. Methyl-and butylparaben had no effect on the expression of selected apoptotic genes in MCF-7. In MCF-10A, all parabens tested increased the expression of G(1)/S phase genes, and downregulated cell cycle inhibitors. Methylparaben increased pro-survival gene. Butylparaben increased BCL2L1 gene, as did 17 beta-estradiol, while propylparaben upregulated both the extrinsic and intrinsic apoptotic pathways. There are differences in cell cycle and apoptosis gene expression between parabens and 17 beta-estradiol in MCF-7 cells. In MCF-10A cells, most of the genes activated by parabens were comparable to those activated by 17 beta-estradiol. Copyright (C) 2014 John Wiley & Sons, Ltd.
机译：羟基苯甲酸是对羟基苯甲酸的烷基酯，广泛用作消费产品中的抗微生物防腐剂，包括药物，食品和化妆品。我们以前展示了甲基 - ，丁基和丙基羟基苯甲酸酯，即使低剂量，也刺激MCF-7乳腺癌细胞和未转化的MCF-10A乳腺上皮细胞的增殖。本研究旨在确定这是否对细胞周期和凋亡基因表达的直接影响。将MCF-7和MCF-10A细胞暴露于甲基，丁基和丙基羟基苯（20nm）或17β-雌二醇（10nm）中。通过使用Western印迹的实时聚合酶链反应和蛋白质表达评估细胞周期和凋亡基因表达。 17β-雌二醇上调的G（1）/ S期基因和MCF-7和MCF-10A中的下调细胞周期进展抑制剂。在MCF-7中观察到Bcl-XL的上调和半胱天蛋酶9的下调，同时在MCF-10a中注意到Bcl-XL，Bcl2L2和Caspase 9的上调。 MCF-7细胞中的细胞环蛋白不受任何羟基苯甲丝的影响。甲基 - 和丁基羟基苯甲酸对MCF-7中选择的凋亡基因的表达没有影响。在MCF-10A中，所有的羟基苯甲酸酯测试增加了G（1）/ S期基因的表达和下调的细胞周期抑制剂。甲基羟基甲酸甲基羟基苯甲酸盐增加了促求生存基因。丁基甘氨酸的增加的Bcl2L1基因，如17β-雌二醇，而丙基羟基苯甲酸丙酯上调了外部和固有凋亡途径。在MCF-7细胞中，羟基甲苯和17β-雌二醇之间的细胞周期和凋亡基因表达存在差异。在MCF-10A细胞中，由羟基苯甲酸酯激活的大多数基因与17β-雌二醇激活的基因相当。版权所有（c）2014 John Wiley＆Sons，Ltd。

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