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首页> 外文期刊>Journal of applied toxicology >In chemico skin sensitization risk assessment of botanical ingredients
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In chemico skin sensitization risk assessment of botanical ingredients

机译：在Chemico皮肤致敏风险评估植物成分

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Abstract Skin sensitization risk assessment of botanical ingredients is necessary for consumers' protection and occupational hazard identification. There are currently very few available alternative methods that can assist in the evaluation of complex mixtures. Chemical methods can provide essential information in a timely manner and thus help to reduce the need for in vivo testing, and they can complement and facilitate targeted in vitro assays. In the present work, the applicability of the high‐throughput screening with dansyl cysteamine (DCYA) method for the systematic evaluation of skin sensitization of complex botanicals was explored. Botanical ingredients of four unrelated plant species were obtained and tested with the high‐throughput fluorescence method at three concentrations. To illustrate the minimal matrix effects of the tested extracts on the developed method, the least DCYA‐reactive extract ( Rosa canina ) was spiked with known sensitizers at different concentrations. The data obtained from the four plant extracts and the spiking experiments with known sensitizers, suggest that the high‐throughput screening–DCYA method can be successfully applied for estimating the skin sensitization potential of complex botanical matrices. This is the first report of an attempt to develop a versatile in chemico method for the rapid detection of reactive skin sensitizers in complex botanical extracts, which could complement the battery of existing validated, non‐animal methods.
机译：摘要消费者保护和职业危害识别是植物成分的摘要皮肤致敏风险评估。目前还有很少的可用替代方法，可以帮助评估复杂混合物。化学方法可以及时提供必要的信息，从而有助于减少体内测试的需要，并且它们可以补充和促进靶向体外测定。在本作工作中，探讨了高通量筛选用丹甘蓝胱胺（DCYA）方法的适用性，用于系统评价复合植物的皮肤致敏。获得了四种无关植物物种的植物成分，并在三种浓度下用高通量荧光法试验。为了说明测试的提取物对开发方法的最小基质效应，在不同浓度下用已知的敏化剂掺入最少的DCYA反应提取物（Rosa Caniana）。从四种植物提取物和具有已知敏化剂的尖峰实验获得的数据表明，可以成功地应用高通量筛选-DCYA方法以估计复杂植物基质的皮肤敏化电位。这是第一个试图在复合植物提取物中快速检测的Chemico方法开发化学方法的多功能，这可以补充现有验证的非动物方法的电池。

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